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Title: An improved biosensor for acetaldehyde determination using a bienzymatic strategy at poly(neutral red) modified carbon film electrodes
Authors: Ghica, Mariana Emilia 
Pauliukaite, Rasa 
Marchand, Nicolas 
Devic, Eric 
Brett, Christopher M. A. 
Keywords: Nicotinamide adenine dinucleotide oxidase; Aldehyde dehydrogenase; Carbon film electrode; Poly(neutral red); Enzyme cross-linking; Sol-gel
Issue Date: 2007
Citation: Analytica Chimica Acta. 591:1 (2007) 80-86
Abstract: Improved biosensors for acetaldehyde determination have been developed using a bienzymatic strategy, based on a mediator-modified carbon film electrode and co-immobilisation of NADH oxidase and aldehyde dehydrogenase. Modification of the carbon film electrode with poly(neutral red) mediator resulted in a sensitive, low-cost and reliable NADH detector. Immobilisation of the enzymes was performed using encapsulation in a sol-gel matrix or cross-linking with glutaraldehyde. The bienzymatic biosensors were characterized by studying the influence of pH, applied potential and co-factors. The sol-gel and glutaraldehyde biosensors showed a linear response up to 60 [mu]M and 100 [mu]M, respectively, with detection limits of 2.6 [mu]M and 3.3 [mu]M and sensitivities were 1.7 [mu]A mM-1 and 5.6 [mu]A mM-1. The optimised biosensors showed good stability and good selectivity and have been tested for application for the determination of acetaldehyde in natural samples such as wine.
DOI: 10.1016/j.aca.2007.03.047
Rights: openAccess
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais

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