Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/5045
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dc.contributor.authorGhica, Mariana Emilia-
dc.contributor.authorPauliukaite, Rasa-
dc.contributor.authorMarchand, Nicolas-
dc.contributor.authorDevic, Eric-
dc.contributor.authorBrett, Christopher M. A.-
dc.date.accessioned2008-09-01T15:03:00Z-
dc.date.available2008-09-01T15:03:00Z-
dc.date.issued2007en_US
dc.identifier.citationAnalytica Chimica Acta. 591:1 (2007) 80-86en_US
dc.identifier.urihttp://hdl.handle.net/10316/5045-
dc.description.abstractImproved biosensors for acetaldehyde determination have been developed using a bienzymatic strategy, based on a mediator-modified carbon film electrode and co-immobilisation of NADH oxidase and aldehyde dehydrogenase. Modification of the carbon film electrode with poly(neutral red) mediator resulted in a sensitive, low-cost and reliable NADH detector. Immobilisation of the enzymes was performed using encapsulation in a sol-gel matrix or cross-linking with glutaraldehyde. The bienzymatic biosensors were characterized by studying the influence of pH, applied potential and co-factors. The sol-gel and glutaraldehyde biosensors showed a linear response up to 60 [mu]M and 100 [mu]M, respectively, with detection limits of 2.6 [mu]M and 3.3 [mu]M and sensitivities were 1.7 [mu]A mM-1 and 5.6 [mu]A mM-1. The optimised biosensors showed good stability and good selectivity and have been tested for application for the determination of acetaldehyde in natural samples such as wine.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6TF4-4NBH223-4/1/df3a7ae65fbb5f9739654441b2c733eden_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectNicotinamide adenine dinucleotide oxidaseen_US
dc.subjectAldehyde dehydrogenaseen_US
dc.subjectCarbon film electrodeen_US
dc.subjectPoly(neutral red)en_US
dc.subjectEnzyme cross-linkingen_US
dc.subjectSol-gelen_US
dc.titleAn improved biosensor for acetaldehyde determination using a bienzymatic strategy at poly(neutral red) modified carbon film electrodesen_US
dc.typearticleen_US
dc.identifier.doi10.1016/j.aca.2007.03.047-
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1en-
crisitem.author.researchunitCEMMPRE - Centre for Mechanical Engineering, Materials and Processes-
crisitem.author.orcid0000-0002-1590-0816-
crisitem.author.orcid0000-0002-1972-4434-
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais
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