Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/45103
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dc.contributor.authorDuarte, Iola F.-
dc.contributor.authorLamego, Inês-
dc.contributor.authorMarques, Joana-
dc.contributor.authorMarques, M. Paula M.-
dc.contributor.authorBlaise, Benjamin J.-
dc.contributor.authorGil, Ana M.-
dc.date.accessioned2017-12-15T17:56:10Z-
dc.date.available2017-12-15T17:56:10Z-
dc.date.issued2010-
dc.identifier10.1021/pr100635n-
dc.identifier.urihttp://hdl.handle.net/10316/45103-
dc.description.abstractIn the present study, (1)H HRMAS NMR spectroscopy was used to assess the changes in the intracellular metabolic profile of MG-63 human osteosarcoma (OS) cells induced by the chemotherapy agent cisplatin (CDDP) at different times of exposure. Multivariate analysis was applied to the cells spectra, enabling consistent variation patterns to be detected and drug-specific metabolic effects to be identified. Statistical recoupling of variables (SRV) analysis and spectral integration enabled the most relevant spectral changes to be evaluated, revealing significant time-dependent alterations in lipids, choline-containing compounds, some amino acids, polyalcohols, and nitrogenated bases. The metabolic relevance of these compounds in the response of MG-63 cells to CDDP treatment is discussed.por
dc.language.isoengpor
dc.rightsopenAccesspor
dc.subjectAlcoholspor
dc.subjectAmino Acidspor
dc.subjectCholinepor
dc.subjectCisplatinpor
dc.subjectHumanspor
dc.subjectKineticspor
dc.subjectLipidspor
dc.subjectMagnetic Resonance Spectroscopypor
dc.subjectMetabolomepor
dc.subjectOsteosarcomapor
dc.titleNuclear Magnetic Resonance (NMR) Study of the Effect of Cisplatin on the Metabolic Profile of MG-63 Osteosarcoma Cellspor
dc.typearticle-
degois.publication.firstPage5877por
degois.publication.lastPage5886por
degois.publication.issue11por
degois.publication.titleJournal of Proteome Researchpor
dc.peerreviewedyespor
dc.identifier.doi10.1021/pr100635npor
degois.publication.volume9por
item.fulltextCom Texto completo-
item.languageiso639-1en-
item.grantfulltextopen-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
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