Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/3871
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dc.contributor.authorLayden, Brian T.-
dc.contributor.authorAbukhdeir, Abde M.-
dc.contributor.authorWilliams, Nicole-
dc.contributor.authorFonseca, Carla P.-
dc.contributor.authorCarroll, Laura-
dc.contributor.authorCastro, Margarita M. C. A.-
dc.contributor.authorGeraldes, Carlos F. G. C.-
dc.contributor.authorBryant, Fred B.-
dc.contributor.authorFreitas, Duarte Mota de-
dc.date.accessioned2008-08-29T15:35:22Z-
dc.date.available2008-08-29T15:35:22Z-
dc.date.issued2003en_US
dc.identifier.citationBiochemical Pharmacology. 66:10 (2003) 1915-1924en_US
dc.identifier.urihttp://hdl.handle.net/10316/3871-
dc.description.abstractLi+/Mg2+ competition has been implicated in the therapeutic action of Li+ treatment in bipolar illness. We hypothesized that this competition depended on cell-specific properties. To test this hypothesis, we determined the degree of Li+ transport, immobilization, and Li+/Mg2+ competition in lymphoblastomas, neuroblastomas, and erythrocytes. During a 50 mmol/L Li+-loading incubation, Li+ accumulation at 30 min (mmoles Li+/L cells) was the greatest in lymphoblastomas (11.1±0.3), followed by neuroblastomas (9.3±0.5), and then erythrocytes (4.0±0.5). Li+ binding affinities to the plasma membrane in all three cell types were of the same order of magnitude; however, Li+ immobilization in intact cells was greatest in neuroblastomas and least in erythrocytes. When cells were loaded for 30 min in a 50 mmol/L Li+-containing medium, the percentage increase in free intracellular [Mg2+] in neuroblastoma and lymphoblastoma cells (~55 and ~52%, respectively) was similar, but erythrocytes did not exhibit any substantial increase (~6%). With the intracellular [Li+] at 15 mmol/L, the free intracellular [Mg2+] increased by the greatest amount in neuroblastomas (~158%), followed by lymphoblastomas (~75%), and then erythrocytes (~50%). We conclude that Li+ immobilization and transport are related to free intracellular [Mg2+] and to the extent of Li+/Mg2+ competition in a cell-specific manner.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6T4P-49JHRTX-1/1/a707c6f5021c0af8d926e0ee947843b8en_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectFluorescenceen_US
dc.subjectAtomic absorptionen_US
dc.subjectNeuroblastomaen_US
dc.subjectLymphoblastomaen_US
dc.titleEffects of Li+ transport and Li+ immobilization on Li+/Mg2+ competition in cells: implications for bipolar disorderen_US
dc.typearticleen_US
dc.identifier.doi10.1016/j.bcp.2003.07.001-
uc.controloAutoridadeSim-
item.grantfulltextopen-
item.fulltextCom Texto completo-
item.openairetypearticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.languageiso639-1en-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.researchunitCQC - Coimbra Chemistry Centre-
crisitem.author.researchunitCQC - Coimbra Chemistry Centre-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.orcid0000-0001-6811-3878-
crisitem.author.orcid0000-0002-0837-8329-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
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