Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/3871
DC Field | Value | Language |
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dc.contributor.author | Layden, Brian T. | - |
dc.contributor.author | Abukhdeir, Abde M. | - |
dc.contributor.author | Williams, Nicole | - |
dc.contributor.author | Fonseca, Carla P. | - |
dc.contributor.author | Carroll, Laura | - |
dc.contributor.author | Castro, Margarita M. C. A. | - |
dc.contributor.author | Geraldes, Carlos F. G. C. | - |
dc.contributor.author | Bryant, Fred B. | - |
dc.contributor.author | Freitas, Duarte Mota de | - |
dc.date.accessioned | 2008-08-29T15:35:22Z | - |
dc.date.available | 2008-08-29T15:35:22Z | - |
dc.date.issued | 2003 | en_US |
dc.identifier.citation | Biochemical Pharmacology. 66:10 (2003) 1915-1924 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/3871 | - |
dc.description.abstract | Li+/Mg2+ competition has been implicated in the therapeutic action of Li+ treatment in bipolar illness. We hypothesized that this competition depended on cell-specific properties. To test this hypothesis, we determined the degree of Li+ transport, immobilization, and Li+/Mg2+ competition in lymphoblastomas, neuroblastomas, and erythrocytes. During a 50 mmol/L Li+-loading incubation, Li+ accumulation at 30 min (mmoles Li+/L cells) was the greatest in lymphoblastomas (11.1±0.3), followed by neuroblastomas (9.3±0.5), and then erythrocytes (4.0±0.5). Li+ binding affinities to the plasma membrane in all three cell types were of the same order of magnitude; however, Li+ immobilization in intact cells was greatest in neuroblastomas and least in erythrocytes. When cells were loaded for 30 min in a 50 mmol/L Li+-containing medium, the percentage increase in free intracellular [Mg2+] in neuroblastoma and lymphoblastoma cells (~55 and ~52%, respectively) was similar, but erythrocytes did not exhibit any substantial increase (~6%). With the intracellular [Li+] at 15 mmol/L, the free intracellular [Mg2+] increased by the greatest amount in neuroblastomas (~158%), followed by lymphoblastomas (~75%), and then erythrocytes (~50%). We conclude that Li+ immobilization and transport are related to free intracellular [Mg2+] and to the extent of Li+/Mg2+ competition in a cell-specific manner. | en_US |
dc.description.uri | http://www.sciencedirect.com/science/article/B6T4P-49JHRTX-1/1/a707c6f5021c0af8d926e0ee947843b8 | en_US |
dc.format.mimetype | aplication/PDF | en |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.subject | Fluorescence | en_US |
dc.subject | Atomic absorption | en_US |
dc.subject | Neuroblastoma | en_US |
dc.subject | Lymphoblastoma | en_US |
dc.title | Effects of Li+ transport and Li+ immobilization on Li+/Mg2+ competition in cells: implications for bipolar disorder | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1016/j.bcp.2003.07.001 | - |
uc.controloAutoridade | Sim | - |
item.fulltext | Com Texto completo | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.openairetype | article | - |
item.cerifentitytype | Publications | - |
crisitem.author.researchunit | CQC - Coimbra Chemistry Centre | - |
crisitem.author.researchunit | CQC - Coimbra Chemistry Centre | - |
crisitem.author.parentresearchunit | Faculty of Sciences and Technology | - |
crisitem.author.parentresearchunit | Faculty of Sciences and Technology | - |
crisitem.author.orcid | 0000-0001-6811-3878 | - |
crisitem.author.orcid | 0000-0002-0837-8329 | - |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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filef8947563fb734aa8bd890120d23d6590.pdf | 230.56 kB | Adobe PDF | View/Open |
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