Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/27757
Title: Carbon dots from tryptophan doped glucose for peroxynitrite sensing
Authors: Simões, Eliana F. C. 
Silva, Joaquim C. G. Esteves da 
Leitão, João M. M. 
Keywords: Carbon dots; Tryptophan; Peroxynitrite; Sensing; Fluorescence quenching
Issue Date: 10-Dec-2014
Publisher: Elsevier
Citation: SIMÕES, Eliana F. C.; SILVA, Joaquim C. G. Esteves da; LEITÃO, João M. M. - Carbon dots from tryptophan doped glucose for peroxynitrite sensing. "Analytica Chimica Acta". ISSN 0003-2670. Vol. 852 (2014) p. 174–180
Serial title, monograph or event: Analytica Chimica Acta
Volume: 852
Abstract: Tryptophan doped carbon dots (Trp-CD) were microwave synthesized. The optimum conditions of synthesizing of the Trp-CD were established by response surface multivariate optimization methodologies and were the following: 2.5 g of glucose and 300 mg of tryptophan diluted in 15 mL of water exposed for 5 min to a microwave radiation of 700 W. Trp-CD have an average size of 20 nm, were fluorescent with a quantum yield of 12.4% and the presence of peroxynitrite anion (ONOO−) provokes quenching of the fluorescence. The evaluated analytical methodology for ONOO− detection shows a linear response range from 5 to 25 μM with a limit of detection of 1.5 μM and quantification of 4.9 μM. The capability of the ONOO− quantification was evaluated in standard solutions and in fortified serum samples.
URI: http://hdl.handle.net/10316/27757
ISSN: 0003-2670
DOI: 10.1016/j.aca.2014.08.050
Rights: openAccess
Appears in Collections:FFUC- Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat
Carbon dots from tryptophan doped glucose.pdf2.93 MBAdobe PDFView/Open
Show full item record

SCOPUSTM   
Citations

28
checked on May 29, 2020

WEB OF SCIENCETM
Citations

27
checked on Sep 14, 2020

Page view(s) 50

415
checked on Sep 28, 2020

Download(s) 20

1,196
checked on Sep 28, 2020

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.