Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/27752
Title: Multidetection of antibiotics in liver tissue by Ultra-High-Pressure-Liquid-Chromatography tandem Mass Spectrometry
Authors: Freitas, Andreia 
Barbosa, Jorge 
Ramos, Fernando 
Keywords: Antibiotics; Multiclass; Multidetection; UHPLC-MS/MS; Liver; Validation
Issue Date: Nov-2014
Publisher: Elsevier
Citation: FREITAS, Andreia; BARBOSA, Jorge; RAMOS, Fernando - Multidetection of antibiotics in liver tissue by Ultra-High-Pressure-Liquid-Chromatography tandem Mass Spectrometry. "Journal of Chromatography B". ISSN 1570-0232. (2014)
Serial title, monograph or event: Journal of Chromatography B
Abstract: A multiresidue quantitative screening method covering 39 antibiotics from 7 different families by Ultra-High-Pressure-Liquid-Chromatography tandem Mass Spectrometry (UHPLC-MS/MS) is described. Sulfonamides, trimethoprim, tetracyclines, macrolides, quinolones, penicillins and chloramphenicol are simultaneously detected in liver tissue. A simple sample treatment method consisting of extraction with a mixture of acetonitrile and ethylenediaminetetraacetic acid (EDTA) followed by solid-phase extraction (SPE) with a hydrophilic-lipophilic balanced (HLB) cartridge was developed. The methodology was validated, in accordance with Decision 2002/657/EC, by evaluating the following required parameters: decision limit (CCα), detection capability (CCβ), specificity, repeatability and reproducibility. The precision, in terms of the relative standard deviation, was under 22% for all of the compounds, and the recoveries were between 80% and 110%. The CCα and CCβ were determined according to the maximum residue limit (MRL) or the minimum required performance limit (MRPL), when established.
URI: https://hdl.handle.net/10316/27752
ISSN: 1570-0232
DOI: 10.1016/j.jchromb.2014.11.008
Rights: openAccess
Appears in Collections:FFUC- Artigos em Revistas Internacionais
I&D CEFarmacêuticos - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais

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