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Title: CD8+T cell profiles in patients with rheumatoid arthritis and their relationship to disease activity
Authors: Carvalheiro, Helena 
Duarte, Cátia 
Silva-Cardoso, Sandra 
Silva, José A. P. da 
Souto-Carneiro, M. Margarida 
Keywords: CD8+T cells in RA
Issue Date: 2014
Publisher: American College of Rheumatology
Citation: CARVALHEIRO, Helena [et. al] - CD8+T cell profiles in patients with rheumatoid arthritis and their relationship to disease activity. "Arthritis & Rheumatology". ISSN 2326-5205. (2014)
Serial title, monograph or event: Arthritis & Rheumatology
Abstract: Objective CD8+T cells are abundant in rheumatoid arthritis (RA). However, their role in the disease pathogenesis is poorly defined. Here we investigated the relationship between disease activity and CD8+T cell phenotypes, production of cytokines and cytotoxic molecules in RA peripheral blood (PB) and synovial fluid (SF). Methods CD8+T cell phenotypes were determined in 96 patients with RA (44 in remission, 34 with active disease) and in 64 gender and age-matched healthy controls (HC). Ten paired PB and SF samples from patients with active RA were analyzed. The expression of surface markers, cytokines and proteolytic enzymes in CD8+T cells was evaluated using flow-cytometry. Results The PB CD8+T cells from RA patients with active disease exhibited an effector CD27-CD62L- (p=0.005) phenotype with elevated proinflammatory cytokine expression (TNF-α, IFN-γ, IL-6, IL-17A) when compared to HC. The phenotype observed in patients with active disease persisted in remission, with a significant increase in the frequency of CD69 (p<0.001) and was associated with lower cytokine production. CD8+T cells from SF expressed more robust effector memory (CD27+CD62L-) and activated (CD69+) profiles compared with paired blood-derived subsets. Cytokine-production (IL-6, IL-17A, and IFN-γ) by CD8+T cells from PB and SF was positively correlated within individual donors. The production of cytokines (TNF-α, IFN-γ, IL-17A) by CD8+T cells in the PB from RA patients positively correlated with DAS28. Conclusion Herein we characterize the activation status and proinflammatory potential of CD8+T cells subsets in RA patients. This activation status strongly suggests a local and systemic effector cytotoxic role in the disease.
ISSN: 2326-5205
DOI: 10.1002/art.38941
Rights: embargoedAccess
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais

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