Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10316/27565
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Carvalheiro, Helena | - |
dc.contributor.author | Duarte, Cátia | - |
dc.contributor.author | Silva-Cardoso, Sandra | - |
dc.contributor.author | Silva, José A. P. da | - |
dc.contributor.author | Souto-Carneiro, M. Margarida | - |
dc.date.accessioned | 2014-11-10T15:35:43Z | - |
dc.date.available | 2015-11-11T03:00:07Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | CARVALHEIRO, Helena [et. al] - CD8+T cell profiles in patients with rheumatoid arthritis and their relationship to disease activity. "Arthritis & Rheumatology". ISSN 2326-5205. (2014) | por |
dc.identifier.issn | 2326-5205 | - |
dc.identifier.uri | https://hdl.handle.net/10316/27565 | - |
dc.description.abstract | Objective CD8+T cells are abundant in rheumatoid arthritis (RA). However, their role in the disease pathogenesis is poorly defined. Here we investigated the relationship between disease activity and CD8+T cell phenotypes, production of cytokines and cytotoxic molecules in RA peripheral blood (PB) and synovial fluid (SF). Methods CD8+T cell phenotypes were determined in 96 patients with RA (44 in remission, 34 with active disease) and in 64 gender and age-matched healthy controls (HC). Ten paired PB and SF samples from patients with active RA were analyzed. The expression of surface markers, cytokines and proteolytic enzymes in CD8+T cells was evaluated using flow-cytometry. Results The PB CD8+T cells from RA patients with active disease exhibited an effector CD27-CD62L- (p=0.005) phenotype with elevated proinflammatory cytokine expression (TNF-α, IFN-γ, IL-6, IL-17A) when compared to HC. The phenotype observed in patients with active disease persisted in remission, with a significant increase in the frequency of CD69 (p<0.001) and was associated with lower cytokine production. CD8+T cells from SF expressed more robust effector memory (CD27+CD62L-) and activated (CD69+) profiles compared with paired blood-derived subsets. Cytokine-production (IL-6, IL-17A, and IFN-γ) by CD8+T cells from PB and SF was positively correlated within individual donors. The production of cytokines (TNF-α, IFN-γ, IL-17A) by CD8+T cells in the PB from RA patients positively correlated with DAS28. Conclusion Herein we characterize the activation status and proinflammatory potential of CD8+T cells subsets in RA patients. This activation status strongly suggests a local and systemic effector cytotoxic role in the disease. | por |
dc.language.iso | eng | por |
dc.publisher | American College of Rheumatology | por |
dc.rights | embargoedAccess | por |
dc.subject | CD8+T cells in RA | por |
dc.title | CD8+T cell profiles in patients with rheumatoid arthritis and their relationship to disease activity | por |
dc.type | article | por |
degois.publication.title | Arthritis & Rheumatology | por |
dc.relation.publisherversion | http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)2326-5205 | por |
dc.peerreviewed | Yes | por |
dc.identifier.doi | 10.1002/art.38941 | - |
item.fulltext | Com Texto completo | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.openairetype | article | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
crisitem.author.orcid | 0000-0002-2782-6780 | - |
Aparece nas coleções: | I&D CNC - Artigos em Revistas Internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
CELL PROFILES IN PATIENTS.pdf | 2.21 MB | Adobe PDF | Ver/Abrir |
Citações SCOPUSTM
63
Visto em 15/jul/2024
Citações WEB OF SCIENCETM
5
60
Visto em 2/jul/2024
Visualizações de página 50
444
Visto em 23/jul/2024
Downloads 50
540
Visto em 23/jul/2024
Google ScholarTM
Verificar
Altmetric
Altmetric
Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.