Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/26914
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Santos, Ricardo M. | - |
dc.contributor.author | Rodrigues, Marcelo S. | - |
dc.contributor.author | Laranjinha, João | - |
dc.contributor.author | Barbosa, Rui M. | - |
dc.date.accessioned | 2014-09-24T11:07:04Z | - |
dc.date.available | 2014-09-24T11:07:04Z | - |
dc.date.issued | 2013-06-15 | - |
dc.identifier.citation | SANTOS, Ricardo M. [et al.] - Biomimetic sensor based on hemin/carbon nanotubes/chitosan modified microelectrode for nitric oxide measurement in the brain. "Biosensors and Bioelectronics". ISSN 0956-5663. Vol. 44 (2013) p. 152-159 | por |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | https://hdl.handle.net/10316/26914 | - |
dc.description.abstract | A novel biomimetic microsensor for measuring nitric oxide (NO) in the brain in vivo was developed. The sensor consists of hemin and functionalized multi-wall carbon nanotubes covalently attached to chitosan via the carbodiimide crosslinker EDC followed by chitosan electrodeposition on the surface of carbon fiber microelectrodes. Cyclic voltammetry supported direct electron transfer from the FeIII/FeII couple of hemin to the carbon surface at −0.370 V and −0.305 V vs. Ag/AgCl for cathodic and anodic peaks, respectively. Square wave voltammetry revealed a NO reduction peak at −0.762 V vs. Ag/AgCl that increased linearly with NO concentration between 0.25 and 1 μM. The average sensitivity of the microsensors was 1.72 nA/μM and the limit of detection was 25 nM. Oxygen and hydrogen peroxide reduction peaks were observed at −0.269 V and −0.332 V vs. Ag/AgCl, respectively and no response was observed for other relevant interferents, namely ascorbate, nitrite and dopamine. The microsensor was successfully applied to the measurement of exogenously applied NO in the rat brain in vivo. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier | por |
dc.rights | openAccess | por |
dc.subject | Nitric oxide microsensor | por |
dc.subject | Hemin | por |
dc.subject | Carbon nanotubes | por |
dc.subject | Chitosan | por |
dc.subject | In vivo electrochemistry | por |
dc.title | Biomimetic sensor based on hemin/carbon nanotubes/chitosan modified microelectrode for nitric oxide measurement in the brain | por |
dc.type | article | por |
degois.publication.firstPage | 152 | por |
degois.publication.lastPage | 159 | por |
degois.publication.title | Biosensors and Bioelectronics | por |
dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S0956566313000171 | por |
dc.peerreviewed | Yes | por |
dc.identifier.doi | 10.1016/j.bios.2013.01.015 | - |
degois.publication.volume | 44 | por |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0003-4370-5177 | - |
Appears in Collections: | FFUC- Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Biomimetic Sensor Based on Hemin.pdf | 619.79 kB | Adobe PDF | View/Open |
SCOPUSTM
Citations
63
checked on Nov 9, 2022
WEB OF SCIENCETM
Citations
1
63
checked on May 2, 2023
Page view(s)
398
checked on Oct 16, 2024
Download(s) 50
692
checked on Oct 16, 2024
Google ScholarTM
Check
Altmetric
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.