Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/25793
DC FieldValueLanguage
dc.contributor.authorCardoso, Susana-
dc.contributor.authorSantos, M. S.-
dc.contributor.authorMoreno, A. J. M.-
dc.contributor.authorMoreira, Paula I.-
dc.date.accessioned2014-05-28T10:39:41Z-
dc.date.available2014-05-28T10:39:41Z-
dc.date.issued2013-
dc.identifier.urihttp://hdl.handle.net/10316/25793-
dc.description.abstractA growing body of evidence suggests that mitochondrial proton-leak functions as a regulator of reactive oxygen species production and its modulation may limit oxidative injury to tissues. The main purpose of this work was to characterize the proton-leak of brain cortical mitochondria from long-term hyperglycemic and insulininduced recurrent hypoglycemic rats through the modulation of the uncoupling protein 2 (UCP2) and adenine nucleotide translocator (ANT). Streptozotocin-induced diabetic rats were treated subcutaneously with twice-daily insulin injections during 2 weeks to induce the hypoglycemic episodes. No differences in the basal proton-leak, UCP2 and ANT protein levels were observed between the experimental groups. Mitochondria from recurrent hypoglycemic rats presented a decrease in proton-leak in the presence of GDP, a specific UCP2 inhibitor, while an increase in proton-leak was observed in the presence of linoleic acid, a proton-leak activator, this effect being reverted by the simultaneous addition of GDP. Mitochondria from longterm hyperglycemic rats showed an enhanced susceptibility to ANT modulation as demonstrated by the complete inhibition of basal and linoleic acid-induced proton-leak caused by the ANT specific inhibitor carboxyatractyloside. Our results show that recurrent-hypoglycemia renders mitochondria more susceptible to UCPs modulation while the protonleak of long-term hyperglycemic rats is mainly modulated by ANT, which suggest that brain cortical mitochondria have distinct adaptation mechanisms in face of different metabolic insults.por
dc.description.sponsorshipThe authors’ work is supported by the Fundação para a Ciência e a Tecnologia (FCT) (PTDC/SAU-NEU/103325/2008) co-funded by Fundo Europeu de Desenvolvimento Regional (FEDER) via Programa Operacional Factores de Competitividade (COMPETE). Susana Cardoso has a PhD fellowship from the Portuguese Foundation for Science and Technology (SFRH/BD/43968/2008).por
dc.language.isoengpor
dc.publisherSpringer Sciencepor
dc.rightsopenAccesspor
dc.subjectANTpor
dc.subjectCortical brain mitochondriapor
dc.subjectLong-term hyperglycemiapor
dc.subjectProton-leakpor
dc.subjectRecurrent hypoglycemiapor
dc.subjectUCP2por
dc.titleUCP2 and ANT differently modulate proton-leak in brain mitochondria of long-term hyperglycemic and recurrent hypoglycemic ratspor
dc.typearticlepor
degois.publication.firstPage397por
degois.publication.lastPage407por
degois.publication.issue4por
degois.publication.titleJournal of Bioenergetics and Biomembranespor
dc.relation.publisherversionhttp://link.springer.com/article/10.1007%2Fs10863-013-9503-2por
dc.peerreviewedYespor
dc.identifier.doi10.1007/s10863-013-9503-2-
degois.publication.volume45por
item.languageiso639-1en-
item.fulltextCom Texto completo-
item.grantfulltextopen-
crisitem.author.deptFaculdade de Ciências e Tecnologia, Universidade de Coimbra-
crisitem.author.parentdeptUniversidade de Coimbra-
crisitem.author.researchunitMARE - Marine and Environmental Sciences Centre-
crisitem.author.orcid0000-0003-3575-7604-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
Files in This Item:
File Description SizeFormat
J Bioenerg Biomembr (2013) 45, 397–407.pdf672.89 kBAdobe PDFView/Open
Show simple item record

SCOPUSTM   
Citations

16
checked on Nov 8, 2019

WEB OF SCIENCETM
Citations

15
checked on Nov 7, 2019

Page view(s)

106
checked on Dec 4, 2019

Download(s)

70
checked on Dec 4, 2019

Google ScholarTM

Check

Altmetric

Dimensions


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.