Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/25773
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dc.contributor.authorCarrola, João-
dc.contributor.authorSantos, Nádia-
dc.contributor.authorRocha, Maria J.-
dc.contributor.authorFontainhas-Fernandes, António-
dc.contributor.authorPardal, M. A.-
dc.contributor.authorMonteiro, Rogério A. F.-
dc.contributor.authorRocha, Eduardo-
dc.date.accessioned2014-05-23T11:33:52Z-
dc.date.available2014-05-23T11:33:52Z-
dc.date.issued2014-
dc.identifier.urihttps://hdl.handle.net/10316/25773-
dc.description.abstractFish are bioindicators of water pollution, and an increased rate of their erythrocyte nuclear morphological abnormalities (ENMAs)—and particularly of erythrocyte micronuclei (EMN)—is used as a genotoxicity biomarker. Despite the potential value of ENMAs andMN, there is scarce information about fish captured in Iberian estuaries. This is the case of the Portuguese estuaries of the Mondego, Douro and Ave, suffering from different levels of environmental stress and where chemical surveys have been disclosing significant amounts of certain pollutants. So, the aim of this study was to evaluate genotoxicants impacts and infer about the exposure at those ecosystems, using the greymullet (Mugil cephalus) as bioindicator and considering the type and frequency of nuclear abnormalities of erythrocytes as proxies of genotoxicity. Sampling of mullets was done throughout the year in the important Mondego, Douro and Ave River estuaries (centre and north-western Portugal). The fish (total n=242) were caught in campaigns made in spring–summer and autumn– winter, using nets or fishing rods. The sampled mullets were comparable between locations in terms of the basic biometric parameters. Blood smears were stained with Diff-Quik to assess the frequencies of six types of ENMAs and MN (given per 1,000 erythrocytes). Some basic water physicochemical parameters were recorded to search for fluctuations matching the ENMAs. Overall, the most frequent nucleus abnormality was the polymorphic type, sequentially followed by the blebbed/ lobed/notched, segmented, kidney shaped, vacuolated,MNand binucleated. The total average frequency of the ENMAs ranged from 73‰in the Mondego to 108‰in the Ave. The polymorphic type was typically ≥50 % of the total ENMAs, averaging about 51‰, when considering all three estuaries. The most serious lesion—the MN—in fish from Mondego and Douro had a similar frequency (≈0.38‰), which was significantly lower than that in the Ave (0.75‰). No significant seasonal differences existed as to the MN rates and seasonal differences existed almost only in the Douro, with the higher values in AW. In general, the pattern of ENMAs frequencies was unrelated with the water physicochemical parameters. Considering the data for both the total ENMAs and for each specific abnormality, and bearing in mind that values of MN in fish erythrocytes >0.3‰ usually reflect pollution by genotoxicants, it is suggested that mullets were likely being chronically exposed to such compounds, even in the allegedly less polluted ecosystem (Mondego). Moreover, data supported the following pollution exposure gradient: Mondego<Douro<Ave. The scenario and inferences nicely agree with the published data from chemical monitoring.por
dc.description.sponsorshipThis work was partially supported by the European Regional Development Fund (ERDF) funds through the Competitiveness and Trade Expansion Program (COMPETE), and by National Funds provided by Fundação para a Ciência e a Tecnologia (FCT), via the research projects PTDC/MAR/70436/2006, PEst-C/MAR/LA0015/ 2011, PEst-C/MAR/LA0015/2013, and also by the PhD grant SFRH/BD/25746/2005.por
dc.language.isoengpor
dc.publisherSpringer-Verlagpor
dc.rightsopenAccesspor
dc.subjectFishpor
dc.subjectBloodpor
dc.subjectErythrocytespor
dc.subjectMicronucleipor
dc.subjectToxicologypor
dc.subjectBiomarkerspor
dc.titleFrequency of micronuclei and of other nuclear abnormalities in erythrocytes of the grey mullet from the Mondego, Douro and Ave estuaries—Portugalpor
dc.typearticlepor
degois.publication.firstPage6057por
degois.publication.lastPage6068por
degois.publication.issue9por
degois.publication.titleEnvironmental Science and Pollution Researchpor
dc.relation.publisherversionhttp://link.springer.com/article/10.1007%2Fs11356-014-2537-0por
dc.peerreviewedYespor
dc.identifier.doi10.1007/s11356-014-2537-0-
degois.publication.volume21por
item.grantfulltextopen-
item.fulltextCom Texto completo-
item.openairetypearticle-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
crisitem.author.researchunitCFE - Centre for Functional Ecology - Science for People & the Planet-
crisitem.author.orcid0000-0001-6048-7007-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
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