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Title: | Transition of Plasmodium Sporozoites into Liver Stage- Like Forms Is Regulated by the RNA Binding Protein Pumilio | Authors: | Gomes-Santos, Carina S. S. Braks, Joanna Prudêncio, Miguel Carret, Céline Gomes, Ana Rita Pain, Arnab Feltwell, Theresa Khan, Shahid Waters, Andrew Janse, Chris Mair, Gunnar R. Mota, Maria M. |
Issue Date: | 19-May-2011 | Publisher: | Public Library of Science | Citation: | GOMES-SANTOS, Carina S. S. [et al.] - Transition of Plasmodium Sporozoites into Liver Stage- Like Forms Is Regulated by the RNA Binding Protein Pumilio. "PLoS Pathogens" [em linha]. Vol. 7, nº 5 (2011) e1002046. [Consult. Dia Mês Ano]. Disponível em WWW:<http://hdl.handle.net/10316/15539>. ISSN 1553-7366 | Project: | info:eu-repo/grantAgreement/EC/FP7/242095 | Serial title, monograph or event: | PLoS Pathogens | Volume: | 7 | Issue: | 5 | Abstract: | Many eukaryotic developmental and cell fate decisions that are effected post-transcriptionally involve RNA binding proteins as regulators of translation of key mRNAs. In malaria parasites (Plasmodium spp.), the development of round, non-motile and replicating exo-erythrocytic liver stage forms from slender, motile and cell-cycle arrested sporozoites is believed to depend on environmental changes experienced during the transmission of the parasite from the mosquito vector to the vertebrate host. Here we identify a Plasmodium member of the RNA binding protein family PUF as a key regulator of this transformation. In the absence of Pumilio-2 (Puf2) sporozoites initiate EEF development inside mosquito salivary glands independently of the normal transmission-associated environmental cues. Puf2- sporozoites exhibit genome-wide transcriptional changes that result in loss of gliding motility, cell traversal ability and reduction in infectivity, and, moreover, trigger metamorphosis typical of early Plasmodium intra-hepatic development. These data demonstrate that Puf2 is a key player in regulating sporozoite developmental control, and imply that transformation of salivary gland-resident sporozoites into liver stage-like parasites is regulated by a post-transcriptional mechanism. | URI: | https://hdl.handle.net/10316/15539 | ISSN: | 1553-7366 | DOI: | 10.1371/journal.ppat.1002046 | Rights: | openAccess |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais |
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