Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/12876
DC Field | Value | Language |
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dc.contributor.author | Macedo, I. Queiroz | - |
dc.contributor.author | Faro, Carlos J. | - |
dc.contributor.author | Pires, Euclides M. | - |
dc.date.accessioned | 2010-03-12T12:59:50Z | - |
dc.date.available | 2010-03-12T12:59:50Z | - |
dc.date.issued | 1993-10 | - |
dc.identifier.citation | Journal of Agricultural and Food Chemistry. 41:10 (1993) 1537-1540 | en_US |
dc.identifier.issn | 0021-8561 | - |
dc.identifier.uri | https://hdl.handle.net/10316/12876 | - |
dc.description.abstract | The action of Cynara cardunculus L. protease on whole bovine K-casein, over a 3-h period at pH 6.4, was investigated. RpHPLC of the 3% trichloroacetic acid (TCA)-solublefraction of the K-casein digestion mixture showed three peptide peaks, which were identified by amino acid analysis and N-terminal analysis as the 106-169 fragment [caseinomacropeptide (CMP)]. Upon selective precipitation with 12% TCA, one glycosylated and two nonglycosylated forms of CMP were distinguished. Analysis of the whole digestion mixture showed no additional peptides. The kinetics of hydrolysis of the PhelO5- Met106 bond was studied by spectrofluorometry, using fluorescein isothiocyanate-labeled K-casein (FTC- K-casein). The values obtained for kat, k, and k were 1.04 s-l, 0.16 pM, and 6.5 pM-l s-l, respectively. The proteolytic coefficient is of the same order of magnitude as those obtained for other milk-clotting enzymes, but the k, is significantly lower, which reflects the higher affinity of Cynara protease to K-casein | en_US |
dc.language.iso | eng | en_US |
dc.publisher | American Chemical Society | en_US |
dc.rights | openAccess | en_US |
dc.title | Specificity and kinetics of the milk-clotting enzyme from cardoon (Cynara cardunculus L.) toward bovine .kappa.-casein | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1021/jf00034a001 | - |
item.fulltext | Com Texto completo | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.languageiso639-1 | en | - |
item.openairetype | article | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-0480-8379 | - |
crisitem.author.orcid | 0000-0002-5853-0165 | - |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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Specificity and kinetics of the milk-clotting enzyme.pdf | 489.63 kB | Adobe PDF | View/Open |
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