Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/12862
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Baldeiras, Inês E. | - |
dc.contributor.author | Santos, Rosa M. | - |
dc.contributor.author | Rosário, Luís M. | - |
dc.date.accessioned | 2010-03-12T09:25:45Z | - |
dc.date.available | 2010-03-12T09:25:45Z | - |
dc.date.issued | 2006 | - |
dc.identifier.citation | Biological Research. 39:3 (2006) 531-539 | en_US |
dc.identifier.issn | 0716-9760 | - |
dc.identifier.uri | https://hdl.handle.net/10316/12862 | - |
dc.description.abstract | Thymeleatoxin (TMX), an activator of Ca2+-sensitive protein kinase C (cPKC) isoforms, was used to assess the PKC isoform specificity of cholinergic potentiation of glucose (11 mM)-induced pulsatile 5-HT/insulin release (PIR) from single mouse pancreatic islets. TMX (100 nM) and carbachol (Cch, 50 \u03bcM) enhanced PIR ~ 3-fold while reducing the underlying [Ca2+]i oscillations (duration and amplitude) by ~ 40-50%. Both effects were ablated by the specific PKC inhibitor bisindolylmaleimide and chronic TMX pretreatment. Cch also evoked an initial transient [Ca2+]i rise and surge of 5-HT release, which remained unaffected by chronic TMX pretreatment. It is concluded that the immediate cholinergic responses are insensitive to cPKC. In contrast, specific activation of a cPKC isoform mediates sustained cholinergic potentiation of glucose-induced insulin secretion | en_US |
dc.language.iso | eng | en_US |
dc.publisher | SciELO | en_US |
dc.rights | openAccess | en_US |
dc.subject | Cytosolic free Ca2+ concentration | en_US |
dc.subject | 5-HT amperometry | en_US |
dc.subject | Islet of Langerhans | en_US |
dc.subject | Protein kinase C | en_US |
dc.subject | Pulsatile insulin release | en_US |
dc.subject | Thymeleatoxin | en_US |
dc.title | Protein kinase C isoform specificity of cholinergic potentiation of glucose-induced pulsatile 5-HT/ insulin release from mouse pancreatic islets | en_US |
dc.type | article | en_US |
item.fulltext | Com Texto completo | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.languageiso639-1 | en | - |
item.openairetype | article | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-8106-7308 | - |
crisitem.author.orcid | 0000-0003-0789-8637 | - |
crisitem.author.orcid | 0000-0001-8329-2333 | - |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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Protein kinase C isoform specificity of cholinergic potentiation.pdf | 2 MB | Adobe PDF | View/Open |
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