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Title: Deletion of the neuropeptide Y (NPY) Y1 receptor gene reveals a regulatory role of NPY on catecholamine synthesis and secretion
Authors: Cavadas, Cláudia 
Céfai, Daniel 
Rosmaninho-Salgado, Joana 
Vieira-Coelho, Maria Augusta 
Moura, Eduardo 
Busso, Nathalie 
Pedrazzini, Thierry 
Grand, Daniela 
Rotman, Samuel 
Waeber, Bernard 
Aubert, Jean-François 
Grouzmann, Eric 
Keywords: Adrenal gland; Tyrosine hydroxylase; Y1 knockout mice
Issue Date: 5-Jul-2006
Publisher: National Academy of Sciences
Citation: PNAS. 103:27 (2006) 10497-10502
Abstract: The contribution of neuropeptide Y (NPY), deriving from adrenal medulla, to the adrenosympathetic tone is unknown. We found that in response to NPY, primary cultures of mouse adrenal chromaffin cells secreted catecholamine, and that this effect was abolished in cultures from NPY Y(1) receptor knockout mice (Y(1)-/-). Compared with wild-type mice (Y(1)+/+), the adrenal content and constitutive release of catecholamine were increased in chromaffin cells from Y(1)-/- mice. In resting animals, catecholamine plasma concentrations were higher in Y(1)-/- mice. Comparing the adrenal glands of both genotypes, no differences were observed in the area of the medulla, cortex, and X zone. The high turnover of adrenal catecholamine in Y(1)-/- mice was explained by the enhancement of tyrosine hydroxylase (TH) activity, although no change in the affinity of the enzyme was observed. The molecular interaction between the Y(1) receptor and TH was demonstrated by the fact that NPY markedly inhibited the forskolin-induced luciferin activity in Y(1) receptor-expressing SK-N-MC cells transfected with a TH promoter sequence. We propose that NPY controls the release and synthesis of catecholamine from the adrenal medulla and consequently contributes to the sympathoadrenal tone
ISSN: 0027-8424
DOI: 10.1073/pnas.0600913103
Rights: openAccess
Appears in Collections:FFUC- Artigos em Revistas Internacionais
FCTUC Ciências da Vida - Artigos em Revistas Internacionais

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