Please use this identifier to cite or link to this item:
Title: Quantification and Localization of Intracellular Free Mg2+ in Bovine Chromaffin Cells
Authors: Montezinho, Liliana P. 
Fonseca, Carla P. 
Geraldes, Carlos F. G. C. 
Castro, M. Margarida C. A. 
Issue Date: 2002
Publisher: Hindawi Publishing Corporation
Citation: Metal-Based Drugs. 9:1-2 (2002) 69-80
Abstract: Magnesium is an essential element for all living systems. The quantification of free intracellular Mg(2+) concentration ([Mg(2+)](i)) is of utmost importance since changes in its basal value may be an indication of different pathologies due to abnormalities of Mg(2+) metabolism. In this work we used (31)P NMR and fluorescence spectroscopy to determine the resting [Mg(2+)](i) in bovine chromaffin cells, a neuron-like cellular model, as well as confocal laser scanning microscopy to study the free Mg(2+) spatial distribution in these cells. (31)P NMR spectroscopy did not prove to be effective for the determination of [Mg(2+)](i) in this particular case due to some special morphological and physiological properties of this cell type. A basal [Mg(2+)](i) value of 0.551 +/- 0.008 mM was found for these cells using fluorescence spectroscopy and the Mg(2+)-sensitive probe furaptra; this value falls in the concentration range reported in the literature for neurons from different sources. This technique proved to be an accurate and sensitive tool to determine the [Mg(2+)](i).lntraceilular free Mg(2+) seems to be essentially localized in the nucleus and around it, as shown by confocal microscopy with the Mg(2+)-sensitive probe Magnesium Green. It was not possible to derive any conclusion about free Mg(2+) localization inside the chromaffin granules and/or in the cytoplasm due to the lack of sufficient spatial resolution and to probe compartmentalization
ISSN: 0793-0291
DOI: 10.1155/MBD.2002.69
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat
Quantification and Localization of Intracellular Free.pdf1.48 MBAdobe PDFView/Open
Show full item record

Page view(s)

checked on Dec 3, 2020


checked on Dec 3, 2020

Google ScholarTM




Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.