Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/12766
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Mendes, A. Ferreira | - |
dc.contributor.author | Carvalho, A. Pato | - |
dc.contributor.author | Caramona, M. Margarida | - |
dc.contributor.author | Lopes, M. Celeste | - |
dc.date.accessioned | 2010-03-09T08:57:21Z | - |
dc.date.available | 2010-03-09T08:57:21Z | - |
dc.date.issued | 2001-08 | - |
dc.identifier.citation | Mediators of Inflammation. 10:4 (2001) 209-215 | en_US |
dc.identifier.issn | 0962-9351 | - |
dc.identifier.uri | https://hdl.handle.net/10316/12766 | - |
dc.description.abstract | AIMS: In this work, we studied the mechanisms by which diphenyleneiodonium chloride (DPI) inhibits nitric oxide (NO) synthesis induced by the proinflammatory cytokine interleukin-1beta (IL-1) in bovine articular chondrocytes. To achieve this, we evaluated the ability of DPI to inhibit the expression and activity of the inducible isoform of the NO synthase (iNOS) induced by IL-1. We also studied the ability of DPI to prevent IL-1-induced NF-kappaB activation and reactive oxygen species (ROS) production. RESULTS: Northern and Western blot analysis, respectively, showed that DPI dose-dependently inhibited IL-1-induced iNOS mRNA and protein synthesis in primary cultures of bovine articular chondrocytes. DPI effectively inhibited NO production (IC50=0.03+/-0.004 microM), as evaluated by the method of Griess. Nuclear factor-kappa B (NF-kappaB) activation, as evaluated by electrophoretic mobility shift assay, was inhibited by DPI (1-10 microM) in a dose-dependent manner. IL-1-induced ROS production, as evaluated by measurement of dichlorofluorescein fluorescence, was inhibited by DPI at concentrations that also prevented NF-kappaB activation and iNOS expression. CONCLUSIONS: DPI inhibits IL-1-induced NO production in chondrocytes by two distinct mechanisms: (i) by inhibiting NOS activity, and (ii) by preventing iNOS expression through the blockade of NF-kappaB activation. These results also support the involvement of reactive oxygen species in IL-1-induced NF-kappaB activation and expression of NF-kappaB-dependent genes, such as iNOS | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Taylor & Francis Ltd | en_US |
dc.rights | openAccess | en_US |
dc.subject | Reactive oxygen species | en_US |
dc.subject | Flavonoid-containing enzymes | en_US |
dc.subject | Diphenyleneiodonium chloride | en_US |
dc.subject | Pro-inflammatory cytokines | en_US |
dc.subject | Chondrocyte | en_US |
dc.subject | Arthritis | en_US |
dc.title | Diphenyleneiodonium inhibits NF-kappaB activation and iNOS expression induced by IL-1beta: involvement of reactive oxygen species | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1080/09629350120080401 | - |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0001-5511-7132 | - |
crisitem.author.orcid | 0000-0003-1950-9360 | - |
Appears in Collections: | FFUC- Artigos em Revistas Internacionais FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Diphenyleneiodonium inhibits NF-kappaB activation.pdf | 318.71 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.