Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/12761
Title: Effect of cyclosporin-A on the blood-retinal barrier permeability in streptozotocin-induced diabetes
Authors: Carmo, Anália 
Cunha-Vaz, José G. 
Carvalho, Arsélio P. 
Lopes, Maria Celeste 
Keywords: Blood-retinal barrier (BRB); Nitric oxide; Interleukin-1b (IL-1b); Cyclosporin-A (Cs-A)
Issue Date: 2000
Publisher: Taylor & Francis Ltd
Citation: Mediators of Inflammation. 9:5 (2000) 243-248
Abstract: BACKGROUND: Our previous results showed that in retinas from streptozotocin (STZ)-induced diabetic rats there is an increased level of interleukin-1beta (IL-1beta). This cytokine may be involved in the expression of the inducible isoform of the nitric oxide synthase (iNOS), with consequent synthesis of large amounts of NO and blood-retinal barrier (BRB) breakdown. AIMS: The aim of this work was to examine whether the administration of cyclosporin-A (Cs-A) to STZ-induced diabetic rats inhibits the synthesis of IL-1beta and the expression of the inducible proteins, iNOS and cyclo-oxygenase-2 (COX-2) in retinal cells, and whether the activity of these proteins contribute to BRB breakdown. METHODS: The level of IL-1beta was evaluated by ELISA and the NO production by L-[3H]-citrulline formation. Expression of iNOS and COX-2 proteins was determined by two methods, western blot and immunohistochemistry. The permeability of the BRB was assessed by quantification of the vitreous protein. RESULTS AND DISCUSSION: Our results indicated that the levels of IL-1beta and NO in retinas from Cs-A-treated diabetic rats are significantly reduced, as compared to that in non-treated diabetic rats. The treatment of diabetic rats with Cs-A also significantly inhibited the expression of the inducible proteins, iNOS and COX-2. The evaluation of the vitreous protein content revealed that Cs-A also reduces the BRB permeability. Taken together, these results suggest that the increased production of the inflammatory mediators, IL-1beta and NO, in diabetes may affect the BRB permeability and therefore contribute to the development of diabetic retinopathy
URI: http://hdl.handle.net/10316/12761
ISSN: 0962-9351
DOI: 10.1080/09629350020025764
Rights: openAccess
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
FCTUC Ciências da Vida - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat
Effect of cyclosporin-A on the blood--retinal.pdf398.46 kBAdobe PDFView/Open
Show full item record

WEB OF SCIENCETM
Citations

45
checked on May 2, 2022

Page view(s)

211
checked on Aug 18, 2022

Download(s)

149
checked on Aug 18, 2022

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.