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Title: A (13)C isotopomer kinetic analysis of cardiac metabolism: influence of altered cytosolic redox and [Ca(2+)](o)
Authors: Carvalho, Rui A. 
Rodrigues, Tiago B. 
Zhao, Piyu 
Jeffrey, F. Mark H. 
Malloy, Craig R.
Sherry, A. Dean
Issue Date: Aug-2004
Publisher: The American Physiological Society
Citation: American Journal of Physiology - Heart and Circulatory Physiology. 287 (2004) H889-H895
Abstract: Rat hearts were perfused with mixtures of [3-(13)C]pyruvate and [3-(13)C]lactate (to alter cytosolic redox) at low (0.5 mM) or high (2.5 mM) Ca(2+) concentrations to alter contractility. Hearts were frozen at various times after exposure to these substrates, were extracted, and were then analyzed by (13)C NMR spectroscopy. The time-dependent multiplets observed in the (13)C NMR resonances of glutamate in all hearts and in malate and aspartate in hearts perfused with high-pyruvate/low-lactate concentrations were analyzed using a kinetic model of the tricarboxylic acid (TCA) cycle. The analysis showed that TCA cycle flux (V(TCA)) and exchange flux (V(X)) that involved cycle intermediates were both sensitive to cell redox and altered Ca(2+) concentration, and the ratio of these fluxes (V(X)/V(TCA)) varied >10-fold
ISSN: 0363-6135
DOI: 10.1152/ajpheart.00976.2003
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais

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