Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/114186
DC Field | Value | Language |
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dc.contributor.author | Dinami, Roberto | - |
dc.contributor.author | Pompili, Luca | - |
dc.contributor.author | Petti, Eleonora | - |
dc.contributor.author | Porru, Manuela | - |
dc.contributor.author | D'Angelo, Carmen | - |
dc.contributor.author | Di Vito, Serena | - |
dc.contributor.author | Rizzo, Angela | - |
dc.contributor.author | Campani, Virginia | - |
dc.contributor.author | De Rosa, Giuseppe | - |
dc.contributor.author | Bruna, Alejandra | - |
dc.contributor.author | Serra, Violeta | - |
dc.contributor.author | Mano, Miguel | - |
dc.contributor.author | Giacca, Mauro | - |
dc.contributor.author | Leonetti, Carlo | - |
dc.contributor.author | Ciliberto, Gennaro | - |
dc.contributor.author | Tarsounas, Madalena | - |
dc.contributor.author | Stoppacciaro, Antonella | - |
dc.contributor.author | Schoeftner, Stefan | - |
dc.contributor.author | Biroccio, Annamaria | - |
dc.date.accessioned | 2024-03-26T11:05:29Z | - |
dc.date.available | 2024-03-26T11:05:29Z | - |
dc.date.issued | 2023-01-11 | - |
dc.identifier.issn | 1757-4676 | pt |
dc.identifier.issn | 1757-4684 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/114186 | - |
dc.description.abstract | The telomeric repeat-binding factor 2 (TRF2) is a telomere-capping protein that plays a key role in the maintenance of telomere structure and function. It is highly expressed in different cancer types, and it contributes to cancer progression. To date, anti-cancer strategies to target TRF2 remain a challenge. Here, we developed a miRNA-based approach to reduce TRF2 expression. By performing a high-throughput luciferase screening of 54 candidate miRNAs, we identified miR-182-3p as a specific and efficient post-transcriptional regulator of TRF2. Ectopic expression of miR-182-3p drastically reduced TRF2 protein levels in a panel of telomerase- or alternative lengthening of telomeres (ALT)-positive cancer cell lines. Moreover, miR-182-3p induced DNA damage at telomeric and pericentromeric sites, eventually leading to strong apoptosis activation. We also observed that treatment with lipid nanoparticles (LNPs) containing miR-182-3p impaired tumor growth in triple-negative breast cancer (TNBC) models, including patient-derived tumor xenografts (PDTXs), without affecting mouse survival or tissue function. Finally, LNPs-miR-182-3p were able to cross the blood-brain barrier and reduce intracranial tumors representing a possible therapeutic option for metastatic brain lesions. | pt |
dc.language.iso | eng | pt |
dc.publisher | Springer Nature | pt |
dc.relation | Italian Association for Cancer Research (AIRC # 21579) and Ministry of Health (CO-2019- 12369662) | pt |
dc.relation | Ministry of Health Ricerca Corrente 2022 and intramural grant-in-aid | pt |
dc.relation | AIRC fellowships | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | miR-182-3p | pt |
dc.subject | target therapy | pt |
dc.subject | telomeres | pt |
dc.subject | TRF2 | pt |
dc.subject | triple-negative breast cancer | pt |
dc.subject.mesh | Animals | pt |
dc.subject.mesh | Humans | pt |
dc.subject.mesh | Mice | pt |
dc.subject.mesh | Apoptosis | pt |
dc.subject.mesh | Cell Line, Tumor | pt |
dc.subject.mesh | Gene Expression Regulation, Neoplastic | pt |
dc.subject.mesh | Telomere | pt |
dc.subject.mesh | MicroRNAs | pt |
dc.subject.mesh | Triple Negative Breast Neoplasms | pt |
dc.title | MiR-182-3p targets TRF2 and impairs tumor growth of triple-negative breast cancer | pt |
dc.type | article | - |
degois.publication.firstPage | e16033 | pt |
degois.publication.issue | 1 | pt |
degois.publication.title | EMBO Molecular Medicine | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.15252/emmm.202216033 | pt |
degois.publication.volume | 15 | pt |
dc.date.embargo | 2023-01-11 | * |
uc.date.periodoEmbargo | 0 | pt |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | Com Texto completo | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0003-1922-4824 | - |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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dinami-et-al-2022-mir-182-3p-targets-trf2-and-impairs-tumor-growth-of-triple-negative-breast-cancer.pdf | 5.08 MB | Adobe PDF | View/Open |
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This item is licensed under a Creative Commons License