Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/114156
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Coelho, Margarida | - |
dc.contributor.author | Mahar, Rohit | - |
dc.contributor.author | Belew, Getachew D. | - |
dc.contributor.author | Torres, Alejandra | - |
dc.contributor.author | Barosa, Cristina | - |
dc.contributor.author | Cabral, Fernando | - |
dc.contributor.author | Viegas, Ivan | - |
dc.contributor.author | Gastaldelli, Amalia | - |
dc.contributor.author | Mendes, Vera M. | - |
dc.contributor.author | Manadas, Bruno | - |
dc.contributor.author | Jones, John G. | - |
dc.contributor.author | Merritt, Matthew E. | - |
dc.date.accessioned | 2024-03-22T11:08:52Z | - |
dc.date.available | 2024-03-22T11:08:52Z | - |
dc.date.issued | 2023-02 | - |
dc.identifier.issn | 0952-3480 | pt |
dc.identifier.issn | 1099-1492 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/114156 | - |
dc.description.abstract | Deuterated water (2 H2 O) is a widely used tracer of carbohydrate biosynthesis in both preclinical and clinical settings, but the significant kinetic isotope effects (KIE) of 2 H can distort metabolic information and mediate toxicity. 18 O-water (H2 18 O) has no significant KIE and is incorporated into specific carbohydrate oxygens via well-defined mechanisms, but to date it has not been evaluated in any animal model. Mice were given H2 18 O during overnight feeding and 18 O-enrichments of liver glycogen, triglyceride glycerol (TG), and blood glucose were quantified by 13 C NMR and mass spectrometry (MS). Enrichment of oxygens 5 and 6 relative to body water informed indirect pathway contributions from the Krebs cycle and triose phosphate sources. Compared with mice fed normal chow (NC), mice whose NC was supplemented with a fructose/glucose mix (i.e., a high sugar [HS] diet) had significantly higher indirect pathway contributions from triose phosphate sources, consistent with fructose glycogenesis. Blood glucose and liver TG 18 O-enrichments were quantified by MS. Blood glucose 18 O-enrichment was significantly higher for HS versus NC mice and was consistent with gluconeogenic fructose metabolism. TG 18 O-enrichment was extensive for both NC and HS mice, indicating a high turnover of liver triglyceride, independent of diet. Thus H2 18 O informs hepatic carbohydrate biosynthesis in similar detail to 2 H2 O but without KIE-associated risks. | pt |
dc.language.iso | eng | pt |
dc.publisher | Wiley-Blackwell | pt |
dc.relation | Portuguese Foundation for Science and Technology-European Comission; Research Grant: FCT-FEDER-02/SAICT/2017/028147, UIDB/0453972020, UIDP/04539/2020, LA/P/0058/2020, POCI-01-0145-FEDER- 007440, REEQ/481/QUI/2006, RECI/QEQQFI/ 0168/2012, CENTRO-07-CT62-FEDER- 402-022125, PD/BD/135178/2017; Marie Skłodowska-Curie Grant Agreement No. 722619 (Project FOIE GRAS) and Grant Agreement No. 734719 (mtFOIE GRAS); The National Mass Spectrometry Network (RNEM); Contract: POCI-01-0145-FEDER- 402-022125 (ref.: ROTEIRO/0028/2013); National Institutes of Health; NIH, Grant Numbers: R01-DK105346, R01-DK132254, P41-GM122698, and 5U2C-DK119889; National Science Foundation Cooperative Agreement DMR-1644779. | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | fructose | pt |
dc.subject | gluconeogenesis | pt |
dc.subject | glycogenesis | pt |
dc.subject | isotope shift | pt |
dc.subject | triose phosphates | pt |
dc.subject.mesh | Mice | pt |
dc.subject.mesh | Animals | pt |
dc.subject.mesh | Glucose | pt |
dc.subject.mesh | Gluconeogenesis | pt |
dc.subject.mesh | Water | pt |
dc.subject.mesh | Liver | pt |
dc.subject.mesh | Glycerol | pt |
dc.subject.mesh | Trioses | pt |
dc.subject.mesh | Fructose | pt |
dc.subject.mesh | Phosphates | pt |
dc.subject.mesh | Blood Glucose | pt |
dc.subject.mesh | Liver Glycogen | pt |
dc.title | Enrichment of hepatic glycogen and plasma glucose from H₂18 O informs gluconeogenic and indirect pathway fluxes in naturally feeding mice | pt |
dc.type | article | - |
degois.publication.firstPage | e4837 | pt |
degois.publication.issue | 2 | pt |
degois.publication.title | NMR in Biomedicine | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1002/nbm.4837 | pt |
degois.publication.volume | 36 | pt |
dc.date.embargo | 2023-02-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | Com Texto completo | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-0392-1118 | - |
crisitem.author.orcid | 0000-0003-2589-2212 | - |
crisitem.author.orcid | 0000-0002-4593-673X | - |
crisitem.author.orcid | 0000-0002-2087-4042 | - |
crisitem.author.orcid | 0000-0002-3745-3885 | - |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais I&D CFE - Artigos em Revistas Internacionais FCTUC Química - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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NMR in Biomedicine - 2022 - Coelho - Enrichment of hepatic glycogen and plasma glucose from H 18O informs gluconeogenic and.pdf | 632.29 kB | Adobe PDF | View/Open |
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