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Title: | Mitochondrial and Redox Changes in Periodontitis and Type 2 Diabetes Human Blood Mononuclear Cells | Authors: | Ferreira, Ildete L. Costa, Solange Moraes, Bruno Costa, Ana Fokt, Olga Marinho, Daniela Alves, Vera Baptista, Isabel P. Rego, A. Cristina |
Keywords: | periodontitis; type 2 diabetes mellitus; oxidative stress; mitochondria; glutathione; peripheral blood mononuclear cells | Issue Date: | 18-Jan-2023 | Publisher: | MDPI | Project: | This study was supported by a Projetos de Investigação Interdisciplinar e Translacional (PEPITA, 2019) grant from the Faculty of Medicine, of the University of Coimbra (FMUC), Coimbra, Portugal, and by the European Regional Development Fund (ERDF) through the COMPETE 2020—Operational Programme for Competitiveness and Internationalization and Portuguese national funds via FCT—Fundação para a Ciência e a Tecnologia, Portugal, under project reference UIDB/04539/2020, UIDP/04539/2020 and LA/P/0058/2020. | Serial title, monograph or event: | Antioxidants | Volume: | 12 | Issue: | 2 | Abstract: | Periodontitis (PDT) and type 2 diabetes (T2D) have demonstrated a bidirectional relationship and imbalanced oxidative stress linked to mitochondrial dysfunction. Therefore, we investigated mitochondrial and redox (de)regulation in peripheral blood mononuclear cells (PBMCs) in comorbid T2D-PDT, compared to PDT, T2D patients, and control individuals. PBMCs were analyzed for mitochondrial respiration, reactive oxygen species, antioxidant proteins, and expression of Nrf2-target genes. PDT and T2D-PDT patients exhibited altered periodontal clinical markers, while T2D and T2D-PDT patients displayed increased blood HbA1c. Decreased oxygen consumption and ATP production were observed in the PDT patient's PBMCs. PDT and T2D-PDT PBMCs also evidenced increased H2O2 levels and reduced catalase levels (also detected in T2D patients), whereas a compromised glutathione cycle was observed in T2D-PDT patients. PBMCs from both T2D or T2D-PDT patients showed increased Nrf2 protein levels, enhanced GCL activity and GCL-catalytic subunit protein levels, and maintained GCLc, GST, and HO-1 mRNA levels. In contrast, the expressions of Nrf2-target genes were significantly diminished in the PDT patient's PBMCs. Decreased SOD1 and GST mRNA levels were also observed in CD3+CD8+-lymphocytes derived from PDT and T2D-PDT patients. In conclusion, PBMCs from T2D-PDT patients showed major redox changes, while mononuclear cells from PDT patients showed mitochondrial deregulation and reduced expression of Nrf2-target genes. | URI: | https://hdl.handle.net/10316/114068 | ISSN: | 2076-3921 | DOI: | 10.3390/antiox12020226 | Rights: | openAccess |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais FMUC Medicina - Artigos em Revistas Internacionais IIIUC - Artigos em Revistas Internacionais I&D ICBR - Artigos em Revistas Internacionais |
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