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Title: | Thrombin-Binding Aptamer Quadruplex Formation: AFM and Voltammetric Characterization | Authors: | Diculescu, Victor Constantin Chiorcea-Paquim, Ana Maria Eritja, Ramon Oliveira-Brett, Ana Maria |
Issue Date: | 16-Jun-2010 | Publisher: | Hindawi | Project: | SFRH/BPD/36110/2007 PTDC/QUI/65255/2006 PTDC/ QUI/098562/2008, POCI 2010 CEMUC-R (Research Unit 285) COST ActionMP0802 |
Serial title, monograph or event: | Journal of Nucleic Acids | Volume: | 2010 | Abstract: | The adsorption and the redox behaviour of thrombin-binding aptamer (TBA) and extended TBA (eTBA) were studied using atomic force microscopy and voltammetry at highly oriented pyrolytic graphite and glassy carbon. The different adsorption patterns and degree of surface coverage were correlated with the sequence base composition, presence/absence of K(+), and voltammetric behaviour of TBA and eTBA. In the presence of K(+), only a few single-stranded sequences present adsorption, while the majority of the molecules forms stable and rigid quadruplexes with no adsorption. Both TBA and eTBA are oxidized and the only anodic peak corresponds to guanine oxidation. Upon addition of K(+) ions, TBA and eTBA fold into a quadruplex, causing the decrease of guanine oxidation peak and occurrence of a new peak at a higher potential due to the oxidation of G-quartets. The higher oxidation potential of G-quartets is due to the greater difficulty of electron transfer from the inside of the quadruplex to the electrode surface than electron transfer from the more flexible single strands. | URI: | https://hdl.handle.net/10316/110194 | ISSN: | 2090-021X | DOI: | 10.4061/2010/841932 | Rights: | openAccess |
Appears in Collections: | FCTUC Química - Artigos em Revistas Internacionais |
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Thrombinbinding-aptamer-quadruplex-formation-AFM-and-voltammetric-characterizationJournal-of-Nucleic-Acids.pdf | 7.84 MB | Adobe PDF | View/Open |
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