Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/110194
DC Field | Value | Language |
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dc.contributor.author | Diculescu, Victor Constantin | - |
dc.contributor.author | Chiorcea-Paquim, Ana Maria | - |
dc.contributor.author | Eritja, Ramon | - |
dc.contributor.author | Oliveira-Brett, Ana Maria | - |
dc.date.accessioned | 2023-11-16T12:13:35Z | - |
dc.date.available | 2023-11-16T12:13:35Z | - |
dc.date.issued | 2010-06-16 | - |
dc.identifier.issn | 2090-021X | pt |
dc.identifier.uri | https://hdl.handle.net/10316/110194 | - |
dc.description.abstract | The adsorption and the redox behaviour of thrombin-binding aptamer (TBA) and extended TBA (eTBA) were studied using atomic force microscopy and voltammetry at highly oriented pyrolytic graphite and glassy carbon. The different adsorption patterns and degree of surface coverage were correlated with the sequence base composition, presence/absence of K(+), and voltammetric behaviour of TBA and eTBA. In the presence of K(+), only a few single-stranded sequences present adsorption, while the majority of the molecules forms stable and rigid quadruplexes with no adsorption. Both TBA and eTBA are oxidized and the only anodic peak corresponds to guanine oxidation. Upon addition of K(+) ions, TBA and eTBA fold into a quadruplex, causing the decrease of guanine oxidation peak and occurrence of a new peak at a higher potential due to the oxidation of G-quartets. The higher oxidation potential of G-quartets is due to the greater difficulty of electron transfer from the inside of the quadruplex to the electrode surface than electron transfer from the more flexible single strands. | pt |
dc.language.iso | eng | pt |
dc.publisher | Hindawi | pt |
dc.relation | SFRH/BPD/36110/2007 | pt |
dc.relation | PTDC/QUI/65255/2006 | pt |
dc.relation | PTDC/ QUI/098562/2008, POCI 2010 | pt |
dc.relation | CEMUC-R (Research Unit 285) | pt |
dc.relation | COST ActionMP0802 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.title | Thrombin-Binding Aptamer Quadruplex Formation: AFM and Voltammetric Characterization | pt |
dc.type | article | - |
degois.publication.firstPage | 1 | pt |
degois.publication.lastPage | 8 | pt |
degois.publication.title | Journal of Nucleic Acids | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.4061/2010/841932 | pt |
degois.publication.volume | 2010 | pt |
dc.date.embargo | 2010-06-16 | * |
uc.date.periodoEmbargo | 0 | pt |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.openairetype | article | - |
crisitem.author.researchunit | CEMMPRE - Centre for Mechanical Engineering, Materials and Processes | - |
crisitem.author.researchunit | CEMMPRE - Centre for Mechanical Engineering, Materials and Processes | - |
crisitem.author.researchunit | CEMMPRE - Centre for Mechanical Engineering, Materials and Processes | - |
crisitem.author.orcid | 0000-0003-0719-8016 | - |
crisitem.author.orcid | 0000-0002-0605-5147 | - |
crisitem.author.orcid | 0000-0002-6244-0891 | - |
Appears in Collections: | FCTUC Química - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
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Thrombinbinding-aptamer-quadruplex-formation-AFM-and-voltammetric-characterizationJournal-of-Nucleic-Acids.pdf | 7.84 MB | Adobe PDF | View/Open |
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This item is licensed under a Creative Commons License