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Title: | The apoptogenic toxin AIP56 is a metalloprotease A-B toxin that cleaves NF-κb P65 | Authors: | Silva, Daniela S. Pereira, Liliana M. G. Moreira, Ana R. Ferreira-da-Silva, Frederico Brito, Rui M. Faria, Tiago Q. Zornetta, Irene Montecucco, Cesare Oliveira, Pedro Azevedo, Jorge E. Pereira, Pedro J. B. Macedo-Ribeiro, Sandra do Vale, Ana dos Santos, Nuno M. S. |
Issue Date: | Feb-2013 | Publisher: | Public Library of Science | Project: | Daniela S. Silva and Liliana M. G. Pereira were funded by FCT fellowships SFRH/BD/35865/2007 and SFRH/BD/43501/2008, respectively. Ana do Vale was supported by Programa Cieˆncia – funded by POPH - QREN - Tipologia 4.2 - Promoc¸a˜o do Emprego Cientı´fico, co-funded by Fundo Social Europeu and National funding from MCTES. This work was supported by FCT projects PTDC/BIA-PRO/101111/2008, PTDC/CVT/099544/2008 PTDC/MAR/101143/2008 and PEst-C/SAU/ LA0002/2011 (EU-FEDER funding through COMPETE). | Serial title, monograph or event: | PLoS Pathogens | Volume: | 9 | Issue: | 2 | Abstract: | AIP56 (apoptosis-inducing protein of 56 kDa) is a major virulence factor of Photobacterium damselae piscicida (Phdp), a Gram-negative pathogen that causes septicemic infections, which are among the most threatening diseases in mariculture. The toxin triggers apoptosis of host macrophages and neutrophils through a process that, in vivo, culminates with secondary necrosis of the apoptotic cells contributing to the necrotic lesions observed in the diseased animals. Here, we show that AIP56 is a NF-κB p65-cleaving zinc-metalloprotease whose catalytic activity is required for the apoptogenic effect. Most of the bacterial effectors known to target NF-κB are type III secreted effectors. In contrast, we demonstrate that AIP56 is an A-B toxin capable of acting at distance, without requiring contact of the bacteria with the target cell. We also show that the N-terminal domain cleaves NF-κB at the Cys(39)-Glu(40) peptide bond and that the C-terminal domain is involved in binding and internalization into the cytosol. | URI: | https://hdl.handle.net/10316/109792 | ISSN: | 1553-7374 | DOI: | 10.1371/journal.ppat.1003128 | Rights: | openAccess |
Appears in Collections: | FCTUC Química - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais |
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