Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/109792
DC Field | Value | Language |
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dc.contributor.author | Silva, Daniela S. | - |
dc.contributor.author | Pereira, Liliana M. G. | - |
dc.contributor.author | Moreira, Ana R. | - |
dc.contributor.author | Ferreira-da-Silva, Frederico | - |
dc.contributor.author | Brito, Rui M. | - |
dc.contributor.author | Faria, Tiago Q. | - |
dc.contributor.author | Zornetta, Irene | - |
dc.contributor.author | Montecucco, Cesare | - |
dc.contributor.author | Oliveira, Pedro | - |
dc.contributor.author | Azevedo, Jorge E. | - |
dc.contributor.author | Pereira, Pedro J. B. | - |
dc.contributor.author | Macedo-Ribeiro, Sandra | - |
dc.contributor.author | do Vale, Ana | - |
dc.contributor.author | dos Santos, Nuno M. S. | - |
dc.date.accessioned | 2023-10-27T08:35:54Z | - |
dc.date.available | 2023-10-27T08:35:54Z | - |
dc.date.issued | 2013-02 | - |
dc.identifier.issn | 1553-7374 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/109792 | - |
dc.description.abstract | AIP56 (apoptosis-inducing protein of 56 kDa) is a major virulence factor of Photobacterium damselae piscicida (Phdp), a Gram-negative pathogen that causes septicemic infections, which are among the most threatening diseases in mariculture. The toxin triggers apoptosis of host macrophages and neutrophils through a process that, in vivo, culminates with secondary necrosis of the apoptotic cells contributing to the necrotic lesions observed in the diseased animals. Here, we show that AIP56 is a NF-κB p65-cleaving zinc-metalloprotease whose catalytic activity is required for the apoptogenic effect. Most of the bacterial effectors known to target NF-κB are type III secreted effectors. In contrast, we demonstrate that AIP56 is an A-B toxin capable of acting at distance, without requiring contact of the bacteria with the target cell. We also show that the N-terminal domain cleaves NF-κB at the Cys(39)-Glu(40) peptide bond and that the C-terminal domain is involved in binding and internalization into the cytosol. | pt |
dc.language.iso | eng | pt |
dc.publisher | Public Library of Science | pt |
dc.relation | Daniela S. Silva and Liliana M. G. Pereira were funded by FCT fellowships SFRH/BD/35865/2007 and SFRH/BD/43501/2008, respectively. Ana do Vale was supported by Programa Cieˆncia – funded by POPH - QREN - Tipologia 4.2 - Promoc¸a˜o do Emprego Cientı´fico, co-funded by Fundo Social Europeu and National funding from MCTES. This work was supported by FCT projects PTDC/BIA-PRO/101111/2008, PTDC/CVT/099544/2008 PTDC/MAR/101143/2008 and PEst-C/SAU/ LA0002/2011 (EU-FEDER funding through COMPETE). | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject.mesh | Animals | pt |
dc.subject.mesh | Apoptosis | pt |
dc.subject.mesh | Apoptosis Regulatory Proteins | pt |
dc.subject.mesh | Bacterial Toxins | pt |
dc.subject.mesh | Bass | pt |
dc.subject.mesh | Fish Diseases | pt |
dc.subject.mesh | Host-Pathogen Interactions | pt |
dc.subject.mesh | Leukocytes | pt |
dc.subject.mesh | Metalloproteases | pt |
dc.subject.mesh | Photobacterium | pt |
dc.subject.mesh | Recombinant Proteins | pt |
dc.subject.mesh | Transcription Factor RelA | pt |
dc.subject.mesh | Virulence Factors | pt |
dc.title | The apoptogenic toxin AIP56 is a metalloprotease A-B toxin that cleaves NF-κb P65 | pt |
dc.type | article | - |
degois.publication.firstPage | e1003128 | pt |
degois.publication.issue | 2 | pt |
degois.publication.title | PLoS Pathogens | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1371/journal.ppat.1003128 | pt |
degois.publication.volume | 9 | pt |
dc.date.embargo | 2013-02-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | Com Texto completo | - |
crisitem.author.researchunit | CQC - Coimbra Chemistry Centre | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.parentresearchunit | Faculty of Sciences and Technology | - |
crisitem.author.orcid | 0000-0001-9128-2557 | - |
crisitem.author.orcid | 0000-0003-0564-8415 | - |
Appears in Collections: | FCTUC Química - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais |
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The-Apoptogenic-Toxin-AIP56-Is-a-Metalloprotease-AB-Toxin-that-Cleaves-NFb-P65PLoS-Pathogens.pdf | 2.38 MB | Adobe PDF | View/Open |
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