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Título: | Noninvasive measurement of murine hepatic acetyl-CoA ¹³C-enrichment following overnight feeding with ¹³C-enriched fructose and glucose | Autor: | Carvalho, Filipa Duarte, João Simoes, Ana Rita Cruz, Pedro F. Jones, John G. |
Data: | 2013 | Editora: | Hindawi | Projeto: | The authors acknowledge financial support from the Portuguese Foundation for Science and Technology (research Grants PTDC/EEB-BIO/99810/2008 and PTDC/SAUMET/ 111398/2009). The NMR spectrometers are part of the National NMR Network and were purchased in the framework of the National Programme for Scientific Reequipment, Contract REDE/1517/RMN/2005, with funds from POCI 2010 (FEDER) and the Portuguese Foundation for Science and Technology. | Título da revista, periódico, livro ou evento: | BioMed Research International | Volume: | 2013 | Resumo: | The (13)C-isotopomer enrichment of hepatic cytosolic acetyl-CoA of overnight-fed mice whose drinking water was supplemented with [U-(13)C]fructose, and [1-(13)C]glucose and p-amino benzoic acid (PABA) was quantified by (13)C NMR analysis of urinary N-acetyl-PABA. Four mice were given normal chow plus drinking water supplemented with 5% [1-(13)C]glucose, 2.5% [U-(13)C]fructose, and 2.5% fructose (Solution 1) overnight. Four were given chow and water containing 17.5% [1-(13)C]glucose, 8.75% [U-(13)C]fructose and 8.75% fructose (Solution 2). PABA (0.25%) was present in both studies. Urinary N-acetyl-PABA was analyzed by (13)C NMR. In addition to [2-(13)C]- and [1,2-(13)C]acetyl isotopomers from catabolism of [U-(13)C]fructose and [1-(13)C]glucose to acetyl-CoA, [1-(13)C]acetyl was also found indicating pyruvate recycling activity. This precluded precise estimates of [1-(13)C]glucose contribution to acetyl-CoA while that of [U-(13)C]fructose was unaffected. The fructose contribution to acetyl-CoA from Solutions 1 and 2 was 4.0 ± 0.4% and 10.6 ± 0.6%, respectively, indicating that it contributed to a minor fraction of lipogenic acetyl-CoA under these conditions. | URI: | https://hdl.handle.net/10316/109713 | ISSN: | 2314-6133 2314-6141 |
DOI: | 10.1155/2013/638085 | Direitos: | openAccess |
Aparece nas coleções: | FCTUC Química - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais |
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Noninvasive-measurement-of-murine-hepatic-acetylCoA-C-13-enrichment-following-overnight-feeding-with-C-13-enriched-fructose-and-glucoseBioMed-Research-International.pdf | 1.41 MB | Adobe PDF | Ver/Abrir |
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