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Title: | Antioxidant activity assessment in fruit liquors and spirits: methods comparison | Authors: | Santos, Carolina Botelho, Goreti Caldeira, Ilda Torres, Amílcar Ferreira, Fernanda M. |
Keywords: | fruit liquors and spirits; total antioxidant activity; ABTS; DPPH; total phenolic compounds; licores de frutos e aguardentes; capacidade antioxidante total; ABTS; DPPH; compostos fenólicos totais | Issue Date: | 2014 | Publisher: | Instituto Nacional de Investigacao Agraria e das Pescas | Project: | PTDC/AGR-FOR/3746/2012, “Arbutus unedo plants and products quality improvement for the agro-forest sector” PEst- OE/AGR/UI4033/2014, for CITAB |
Serial title, monograph or event: | Ciencia e Tecnica Vitivinicola | Volume: | 29 | Issue: | 1 | Abstract: | During the last few years, Portugal has seen an increasing trend in the development of new fruit-based products, such as liquors and spirits. The
antioxidant capacity of fruit liquors and spirits, sourced commercially or produced on a pilot-scale, was assessed using two simple
spectrophotometric methods: the 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH)
assays. The major goal of this work was to find which of the two methods is the most suitable and accurate in determining antioxidant capacity for
fruit liquors and spirits. In addition, the total phenolic compounds of both kinds of alcoholic beverages were determined by Folin-Ciocalteau
method. Despite the widespread use of DPPH assay for distilled drinks, a weak Spearman correlation coefficient (r = 0.023) between ABTS and
DPPH methods in spirits were found, as well as, between DPPH values and total phenolic compounds content (r = 0.239). This work highlights
the limitations of DPPH assay when used for analysis of samples with very low antioxidant capacity, as distilled spirits. Portugal, nos últimos anos, tem desenvolvido novos produtos à base de frutos, tais como aguardentes e licores. A capacidade antioxidante dos licores de frutos e aguardentes, obtidos comercialmente ou em produções à escala piloto, foi medida usando dois métodos espetrofotométricos: o 2,2´-azino-bis-(3-etilbenzotiazolina-6-ácido sulfónico) (ABTS) e o 1,1-difenil-2-picrilhidrazil (DPPH). O principal objetivo deste trabalho consistiu em determinar qual o método mais fiável na determinação da capacidade antioxidante total das aguardentes e licores. Adicionalmente, o conteúdo total em compostos fenólicos de ambos os tipos de bebidas alcoólicas foi determinado pelo método de Folin-Ciocalteau. Embora o DPPH seja o método mais utilizado neste tipo de análises, no presente trabalho, no entanto, verificou-se que o coeficiente de correlação de Spearman (r=0.023) entre o ABTS e DPPH é baixo, bem como entre os valores de DPPH e o teor de compostos fenólicos totais (r=0.239). Este trabalho destaca assim as limitações do método de DPPH quando usado em amostras com baixa capacidade antioxidante, como é o caso dos licores de frutos e das aguardentes. |
URI: | https://hdl.handle.net/10316/109388 | ISSN: | 2416-3953 | DOI: | 10.1051/ctv/20142901028 | Rights: | openAccess |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Nacionais |
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