Utilize este identificador para referenciar este registo: https://hdl.handle.net/10316/108194
Título: Extracellular Electrophysiological Measurements of Cooperative Signals in Astrocytes Populations
Autor: Mestre, Ana L. G.
Inácio, Pedro M. C.
Elamine, Youssef
Asgarifar, Sanaz
Lourenço, Ana S.
Cristiano, Maria L. S.
Aguiar, Paulo
Medeiros, Maria C. R. 
Araújo, Inês M. 
Ventura, João André 
Gomes, Henrique L.
Palavras-chave: astrocytes; electrodes; electrophysiology; extracellular; glia; signals
Data: 2017
Editora: Frontiers Media S.A.
Projeto: PTDC/SAU-NMC/112183/2009 
UID/NEU/04539/2013 
UID/BIM/04773/2013 
SFRH/BD/78050/2011 
SFRH/BD/79308/2011 
Título da revista, periódico, livro ou evento: Frontiers in Neural Circuits
Volume: 11
Resumo: Astrocytes are neuroglial cells that exhibit functional electrical properties sensitive to neuronal activity and capable of modulating neurotransmission. Thus, electrophysiological recordings of astroglial activity are very attractive to study the dynamics of glial signaling. This contribution reports on the use of ultra-sensitive planar electrodes combined with low noise and low frequency amplifiers that enable the detection of extracellular signals produced by primary cultures of astrocytes isolated from mouse cerebral cortex. Recorded activity is characterized by spontaneous bursts comprised of discrete signals with pronounced changes on the signal rate and amplitude. Weak and sporadic signals become synchronized and evolve with time to higher amplitude signals with a quasi-periodic behavior, revealing a cooperative signaling process. The methodology presented herewith enables the study of ionic fluctuations of population of cells, complementing the single cells observation by calcium imaging as well as by patch-clamp techniques.
URI: https://hdl.handle.net/10316/108194
ISSN: 1662-5110
DOI: 10.3389/fncir.2017.00080
Direitos: openAccess
Aparece nas coleções:I&D IT - Artigos em Revistas Internacionais
FCTUC Eng.Electrotécnica - Artigos em Revistas Internacionais

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