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https://hdl.handle.net/10316/108188
Title: | Short communication: The effect of changing temperature and agar concentration at proliferation stage in the final success of Aleppo pine somatic embryogenesis | Authors: | Pereira, Cátia Montalbán, Itziar A. Goicoa, Tomás Ugarte, M. Dolores Correia, Sandra Canhoto, Jorge Moncaleán, Paloma |
Keywords: | embryogenic cell lines; Pinus halepensis; somatic embryos; water availability | Issue Date: | 2018 | Publisher: | Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria | Project: | MINECO Spanish Government (project AGL2013-4700-C4-2R) |
Serial title, monograph or event: | Forest Systems | Volume: | 26 | Issue: | 3 | Abstract: | Aim of the study: The effect of physical and chemical conditions at proliferation stage was evaluated in order to elucidate if this stage is the determinant phase to induce a marked effect in Pinus halepensis somatic embryogenesis. Area of study: The study was conducted in research laboratories of Neiker (Arkaute, Spain). Material and methods: Pinus halepensis embryonal masses from ten embryogenic cell lines subjected to nine treatments (tissues cultured at three temperatures on media supplemented with three agar concentrations) at proliferation stage. Main results: Significant differences were observed among different proliferation conditions months later at the end of maturation, germination and acclimatization stages. Research highlights: Aleppo pine embryonal masses are cultured under standard conditions on a culture medium supplemented with 4.5 g/L Gelrite® at 23ºC. However, better results in terms of plantlet production can be obtained proliferating the embryonal masses at 18ºC in a culture media with significantly lower water availability. | URI: | https://hdl.handle.net/10316/108188 | ISSN: | 2171-9845 | DOI: | 10.5424/fs/2017263-11436 | Rights: | openAccess |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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