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Title: Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation
Authors: Lima, A. 
May, G.
Díaz-Colunga, J.
Pedreiro, S.
Paiva, A. 
Ferreira, L. 
Enver, T.
Iborra, F. J.
Neves, R. Pires das 
Issue Date: 8-May-2018
Publisher: Springer Nature
Project: POCI- 01-0145-FEDER-007440 
PTDC/SAUENB/ 113696/2009 
Bloodwise and CRUK program grants to T.E. BFU2013-45918-R and BFU2016-79127-R to F.J.I. 
Serial title, monograph or event: Scientific Reports
Volume: 8
Issue: 1
Abstract: Chromatin structure is a major regulator of transcription and gene expression. Herein we explore the use of osmotic modulation to modify the chromatin structure and reprogram gene expression. In this study we use the extracellular osmotic pressure as a chromatin structure and transcriptional modulator. Hyposmotic modulation promotes chromatin loosening and induces changes in RNA polymerase II (Pol II) activity. The chromatin decondensation opens space for higher amounts of DNA engaged RNA Pol II. Hyposmotic modulation constitutes an alternative route to manipulate cell fate decisions. This technology was tested in model protocols of induced pluripotency and transdifferentiation in cells growing in suspension and adherent to substrates, CD34+ umbilical-cord-blood (UCB), fibroblasts and B-cells. The efficiency and kinetics of these cell fate modulation processes were improved by transient hyposmotic modulation of the cell environment.
ISSN: 2045-2322
DOI: 10.1038/s41598-018-25517-2
Rights: openAccess
Appears in Collections:I&D ICBR - Artigos em Revistas Internacionais
IIIUC - Artigos em Revistas Internacionais
FMUC Medicina - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais

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