Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/107634
DC Field | Value | Language |
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dc.contributor.author | Rodrigues-Neves, Ana C. | - |
dc.contributor.author | Aires, Inês D. | - |
dc.contributor.author | Vindeirinho, Joana R. | - |
dc.contributor.author | Boia, Raquel | - |
dc.contributor.author | Madeira, Maria H. | - |
dc.contributor.author | Gonçalves, Francisco Q. | - |
dc.contributor.author | Cunha, Rodrigo A. | - |
dc.contributor.author | Santos, Paulo | - |
dc.contributor.author | Ambrósio, Francisco | - |
dc.contributor.author | Santiago, Ana R. | - |
dc.date.accessioned | 2023-07-25T10:09:46Z | - |
dc.date.available | 2023-07-25T10:09:46Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 1663-9812 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/107634 | - |
dc.description.abstract | Glaucoma is the second cause of blindness worldwide and is characterized by the degeneration of retinal ganglion cells (RGCs) and optic nerve atrophy. Increased microglia reactivity is an early event in glaucoma that may precede the loss of RGCs, suggesting that microglia and neuroinflammation are involved in the pathophysiology of this disease. Although global changes of the purinergic system have been reported in experimental and human glaucoma, it is not known if this is due to alterations of the purinergic system of microglial cells, the resident immune cells of the central nervous system. We now studied if elevated hydrostatic pressure (EHP), mimicking ocular hypertension, changed the extracellular levels of ATP and adenosine and the expression, density and activity of enzymes, transporters and receptors defining the purinergic system. The exposure of the murine microglial BV-2 cell line to EHP increased the extracellular levels of ATP and adenosine, increased the density of ecto-nucleoside triphosphate diphosphohydrolase 1 (E-NTPDase1, CD39) and decreased the density of the equilibrative nucleotide transporter 2 as well as the activity of adenosine deaminase. The expression of adenosine A1 receptor also decreased, but the adenosine A3 receptor was not affected. Notably, ATP and adenosine selectively control migration rather than phagocytosis, both bolstered by EHP. The results show that the purinergic system is altered in microglia in conditions of elevated pressure. Understanding the impact of elevated pressure on the purinergic system will help to unravel the mechanisms underlying inflammation and neurodegeneration associated with glaucoma. | pt |
dc.language.iso | eng | pt |
dc.publisher | Frontiers Media S.A. | pt |
dc.relation | Project PTDC/BIMMEC/ 0913/2012 | pt |
dc.relation | PEst-C/SAU/UI3282/ 2013 | pt |
dc.relation | UID/NEU/04539/2013 | pt |
dc.relation | FEDER-COMPETE (POCI- 01-0145-FEDER-007440) | pt |
dc.relation | Centro 2020 Regional Operational Programme (CENTRO-01-0145-FEDER-000008: BrainHealth 2020) | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | purinergic system | pt |
dc.subject | ATP | pt |
dc.subject | adenosine | pt |
dc.subject | microglia | pt |
dc.subject | elevated pressure | pt |
dc.subject | glaucoma | pt |
dc.title | Elevated Pressure Changes the Purinergic System of Microglial Cells | pt |
dc.type | article | - |
degois.publication.firstPage | 16 | pt |
degois.publication.issue | JAN | pt |
degois.publication.title | Frontiers in Pharmacology | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3389/fphar.2018.00016 | pt |
degois.publication.volume | 9 | pt |
dc.date.embargo | 2018-01-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | Com Texto completo | - |
crisitem.author.researchunit | ICBR Coimbra Institute for Clinical and Biomedical Research | - |
crisitem.author.researchunit | ICBR Coimbra Institute for Clinical and Biomedical Research | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | ISISE - Institute for Sustainability and Innovation in Structural Engineering | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.parentresearchunit | Faculty of Medicine | - |
crisitem.author.parentresearchunit | Faculty of Medicine | - |
crisitem.author.orcid | 0000-0003-3290-9238 | - |
crisitem.author.orcid | 0000-0001-6282-3553 | - |
crisitem.author.orcid | 0000-0001-8042-0221 | - |
crisitem.author.orcid | 0000-0003-2550-6422 | - |
crisitem.author.orcid | 0000-0002-0134-6762 | - |
crisitem.author.orcid | 0000-0002-0477-1641 | - |
crisitem.author.orcid | 0000-0002-7541-7041 | - |
Appears in Collections: | I&D IBILI - Artigos em Revistas Internacionais FMUC Medicina - Artigos em Revistas Internacionais FCTUC Ciências da Vida - Artigos em Revistas Internacionais I&D ICBR - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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Elevated pressure changes the purinergic system of microglial cells.pdf | 1.75 MB | Adobe PDF | View/Open |
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