Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/106737
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sousa, Maria Inês | - |
dc.contributor.author | Correia, Bibiana | - |
dc.contributor.author | Branco, Ana Filipa | - |
dc.contributor.author | Rodrigues, Ana Sofia | - |
dc.contributor.author | Ramalho-Santos, João | - |
dc.date.accessioned | 2023-04-20T09:32:06Z | - |
dc.date.available | 2023-04-20T09:32:06Z | - |
dc.date.issued | 2020 | - |
dc.identifier.issn | 1687-966X | pt |
dc.identifier.uri | https://hdl.handle.net/10316/106737 | - |
dc.description.abstract | DMSO is a commonly used solvent in biological studies, as it is an amphipathic molecule soluble in both aqueous and organic media. For that reason, it is the vehicle of choice for several water-insoluble substances used in research. At the molecular and cellular level, DMSO is a hydrogen-bound disrupter, an intercellular electrical uncoupler, and a cryoprotectant, among other properties. Importantly, DMSO often has overlooked side effects. In stem cell research, the literature is scarce, but there are reports on the effect of DMSO in human embryoid body differentiation and on human pluripotent stem cell priming towards differentiation, via modulation of cell cycle. However, in mouse embryonic stem cell (mESC) culture, there is almost no available information. Taking into consideration the almost ubiquitous use of DMSO in experiments involving mESCs, we aimed to understand the effect of very low doses of DMSO (0.0001%-0.2%), usually used to introduce pharmacological inhibitors/modulators, in mESCs cultured in two different media (2i and FBS-based media). Our results show that in the E14Tg2a mESC line used in this study, even the smallest concentration of DMSO had minor effects on the total number of cells in serum-cultured mESCs. However, these effects could not be explained by alterations in cell cycle or apoptosis. Furthermore, DMSO did not affect pluripotency or differentiation potential. All things considered, and although control experiments should be carried out in each cell line that is used, it is reasonable to conclude that DMSO at the concentrations used here has a minimal effect on this particular mESC line. | pt |
dc.description.sponsorship | This work was funded by the Fundação para a Ciência e Tecnologia (FCT), Portugal, for the PhD scholarship attributed to M.I.S. (SFRH/BD/86260/2012) and the postdoctoral fellowship attributed to A.S.R. (SFRH/BPD/119367/2016). Funding was also provided by the STEM@REST Project (CENTRO- 01-0145-FEDER-028871) and PAC CANCEL_STEM (POCI-01-0145-FEDER-016390). A.F.B. was hired through the STEM@REST Project (CENTRO-01-0145-FEDER- 028871). Additional funding was provided by the European Regional Development Fund (ERDF) through the Centro 2020 Regional Operational Programme: project CENTRO- 01-0145-FEDER-000012-HealthyAging2020, the COMPETE 2020—Operational Programme for Competitiveness and Internationalisation, and the Portuguese national funds via FCT—Fundação para a Ciência e a Tecnologia, I.P.: project POCI-01-0145-FEDER-007440, that attributed a fellowship to B. C. (BIM-IN0828). | pt |
dc.language.iso | eng | pt |
dc.publisher | Hindawi | pt |
dc.relation | SFRH/BD/86260/2012 | pt |
dc.relation | SFRH/BPD/119367/2016 | pt |
dc.relation | PTDC/BTM-SAL/28871/2017 | pt |
dc.relation | CENTRO-01-0145-FEDER-000012/HealthyAging2020 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.title | Effects of DMSO on the Pluripotency of Cultured Mouse Embryonic Stem Cells (mESCs) | pt |
dc.type | article | - |
degois.publication.firstPage | 8835353 | pt |
degois.publication.title | Stem Cells International | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1155/2020/8835353 | pt |
degois.publication.volume | 2020 | pt |
dc.date.embargo | 2020-01-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-0415-3560 | - |
crisitem.author.orcid | 0000-0001-7060-879X | - |
crisitem.author.orcid | 0000-0002-1172-4018 | - |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Effects-of-DMSO-on-the-Pluripotency-of-Cultured-Mouse-Embryonic-Stem-Cells-mESCsStem-Cells-International.pdf | 3.98 MB | Adobe PDF | View/Open |
SCOPUSTM
Citations
3
checked on Sep 2, 2024
WEB OF SCIENCETM
Citations
2
checked on Jul 2, 2024
Page view(s)
70
checked on Sep 25, 2024
Download(s)
21
checked on Sep 25, 2024
Google ScholarTM
Check
Altmetric
Altmetric
This item is licensed under a Creative Commons License