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Title: | Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics | Authors: | Santos, Ana Isabel Lourenço, Ana Sofia Simão, Sónia Marques da Silva, Dorinda Santos, Daniela Filipa Onofre de Carvalho, Ana Paula Pereira, Ana Catarina Izquierdo-Álvarez, Alicia Ramos, Elena Morato, Esperanza Marina, Anabel Martínez-Ruiz, Antonio Araújo, Inês Maria |
Keywords: | Nitric oxide S-nitrosylation; Neurogenesis; Neural stem cells; Neurogenesis Seizures; Hippocampus | Issue Date: | May-2020 | Publisher: | Elsevier | Project: | SFRH/BD/77903/2011 SFRH/BD/79308/2011 PTDC/QUI-QFI/29319/2017 PTDC/NEU-OSD/0473/2012 UID/BIM/04773/2019 |
Serial title, monograph or event: | Redox Biology | Volume: | 32 | Abstract: | Nitric oxide (NO) is well established as a regulator of neurogenesis. NO increases the proliferation of neural stem cells (NSC), and is essential for hippocampal injury-induced neurogenesis following an excitotoxic lesion. One of the mechanisms underlying non-classical NO cell signaling is protein S-nitrosylation. This post-translational modification consists in the formation of a nitrosothiol group (R-SNO) in cysteine residues, which can promote formation of other oxidative modifications in those cysteine residues. S-nitrosylation can regulate many physiological processes, including neuronal plasticity and neurogenesis. In this work, we aimed to identify S-nitrosylation targets of NO that could participate in neurogenesis. In NSC, we identified a group of proteins oxidatively modified using complementary techniques of thiol redox proteomics. S-nitrosylation of some of these proteins was confirmed and validated in a seizure mouse model of hippocampal injury and in cultured hippocampal stem cells. The identified S-nitrosylated proteins are involved in the ERK/MAPK pathway and may be important targets of NO to enhance the proliferation of NSC. | URI: | https://hdl.handle.net/10316/106654 | ISSN: | 22132317 | DOI: | 10.1016/j.redox.2020.101457 | Rights: | openAccess |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais |
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Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics.pdf | 2.04 MB | Adobe PDF | View/Open |
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