Utilize este identificador para referenciar este registo: https://hdl.handle.net/10316/106368
Título: Cytotoxic Effects of Zoom® Whitening Product in Human Fibroblasts
Autor: Marto, Carlos Miguel 
Laranjo, Mafalda 
Paula, Anabela 
Coelho, Ana Sofia 
Abrantes, Ana Margarida 
Casalta-Lopes, João 
Gonçalves, Ana Cristina 
Sarmento-Ribeiro, Ana Bela 
Ferreira, Manuel Marques 
Cabrita, António 
Botelho, Maria Filomena 
Carrilho, Eunice 
Palavras-chave: tooth whitening; hydrogen peroxide; cytotoxicity; fibroblasts; oxidative stress
Data: 25-Mar-2020
Editora: MDPI
Projeto: UID/NEU/04539/2019 
UIDB/04539/2020 
UIDP/04539/2020 (CIBB) 
POCI-01-0145-FEDER-007440 
Título da revista, periódico, livro ou evento: Materials
Volume: 13
Número: 7
Resumo: Tooth whitening procedures are increasing; however, side effects can occur, such as damage to pulp cells, by the whitening products. This study aims to assess the cellular effects promoted by a whitening product, namely, the oxidative stress fostered by the active agent hydrogen peroxide, with and without photoactivation. Additionally, if cellular recovery occurred, we intended to determine the time point where cells recover from the tooth whitening induced damage. Human fibroblasts were exposed to hydrogen peroxide, Zoom®, Zoom® + irradiation, and irradiation alone. The following analysis was performed: metabolic activity evaluation by the MTT assay; cell viability, mitochondrial membrane potential, peroxides production, superoxide radical production, and reduced glutathione expression by flow cytometry. We determined the IC50 value for all groups, and a dose-dependent cytotoxic effect was verified. At the times analyzed, hydrogen peroxide groups showed no metabolic activity recovery while a cell recovery was observed after 24 h (Zoom®) and 48 h (Zoom® + irradiation). Cell death was seen in hydrogen peroxide and Zoom® + irradiation groups, mainly by apoptosis, and the irradiation had a cytotoxic effect per se. This in vitro study supports that whitening products with moderate hydrogen peroxide (HP) concentration have a temporary effect on cells, allowing a cellular recovery.
URI: https://hdl.handle.net/10316/106368
ISSN: 1996-1944
DOI: 10.3390/ma13071491
Direitos: openAccess
Aparece nas coleções:FMUC Medicina - Artigos em Revistas Internacionais
I&D CIBB - Artigos em Revistas Internacionais
I&D CQC - Artigos em Revistas Internacionais
I&D ICBR - Artigos em Revistas Internacionais

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