Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/105773
Title: Isolation, Identification and Biotechnological Applications of a Novel, Robust, Free-living Chlorococcum (Oophila) amblystomatis Strain Isolated from a Local Pond
Authors: Correia, Nádia 
Pereira, Hugo
Silva, Joana T.
Santos, Tamára
Soares, Maria
Sousa, Carolina B.
Schüler, Lisa M.
Costa, Margarida
Varela, João
Pereira, Leonel 
Silva, Joana
Keywords: bioprospection; microalgae; Chlorococcum (Oophila) amblystomatis; photobioreactors; industrial-scale production; biotechnological applications
Issue Date: 2020
Publisher: MDPI
Project: Portuguese national budget P2020 in the scope of the project no. 023310 – ALGACO2: “Cultivo industrial de microalgas como tecnologia verde para captura de CO2 atmosférico” 
UIDB/04326/2020 
EMBRC.PT ALG-01-0145-FEDER-022121 
BIODATA.PT ALG-01-0145-FEDER-022231 
UIDB/04292/2020 
Serial title, monograph or event: Applied Sciences (Switzerland)
Volume: 10
Issue: 9
Abstract: Bioprospection of novel autochthonous strains is key to the successful industrial-scale production of microalgal biomass. A novel Chlorococcum strain was recently isolated from a pond inside the industrial production facility of Allmicroalgae (Leiria, Portugal). Phylogenetic analysis based on 18S ribosomal ribonucleic acid (rRNA) gene sequences suggests that this isolate is a novel, free-living Oophila amblystomatis strain. However, as our phylogenetic data strongly suggests that the aforementioned taxon belongs to the genus Chlorococcum, it is here proposed to rename this species as Chlorococcum amblystomatis. In order to characterize the biotechnological potential of this novel isolate, growth performance and biochemical composition were evaluated from the pilot (2.5-m3) to industrial (10-m3) scale. The highest maximum areal productivity (36.56 g m􀀀2 day􀀀1) was reached in a 10-m3 tubular photobioreactor (PBR), as compared to that obtained in a 2.5-m3 PBR (26.75 g m􀀀2 day􀀀1). Chlorococcum amblystomatis displayed high protein content (48%–56% dry weight (DW)) and moderate levels of total lipids (18%–31% DW), carbohydrates (6%–18% DW) and ashes (9%–16% DW). Furthermore, the lipid profile was dominated by polyunsaturated fatty acids (PUFAs). The highest pigment contents were obtained in the 2.5-m3 PBR, where total chlorophylls accounted for 40.24 mg g􀀀1 DW, followed by lutein with 5.37 mg g􀀀1 DW. Overall, this free-living Chlorococcum amblystomatis strain shows great potential for nutritional applications, coupling a promising growth performance with a high protein content as well as relevant amounts of PUFAs, chlorophyll, and carotenoids.
URI: https://hdl.handle.net/10316/105773
ISSN: 2076-3417
DOI: 10.3390/app10093040
Rights: openAccess
Appears in Collections:I&D MARE - Artigos em Revistas Internacionais

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