Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/10509
Title: | Effects of negatively charged shift reagents on red blood cell morphology, lithium ion transport, and membrane potential | Authors: | Ramasamy, Ravichandran Freitas, Duarte Mota de Jones, Warren Wezeman, Frederick Labotka, Richard Geraldes, Carlos F. G. C. |
Issue Date: | Oct-1990 | Publisher: | American Chemical Society | Citation: | Inorganic Chemistry. 29:20 (1990) 3979-3985 | Abstract: | Lanthanide shift reagents have been used extensively in multinuclear magnetic resonance (NMR) applications in order to obtain information regarding ion distribution and transport in cellular systems. The aqueous reagents used in this study were Dy(PPP)J-, Tm( PPP)J-, Dy(TTHA)’-, Dy(PcPcP);-, and Dy(DOTP)’-, where Dy3+ and Tm3+ represent dysprosium and thulium ions and PPPs-, TTHA6-, PcPcPs-, and DOTP*- denote the triphosphate, triethylenetetraminehexaacetate, bis(dihydroxyphosphiny1- methyl)phosphinate, and I ,4,7,1 O-tetrazacyclododecane-N,N’,N”,N”’-tetrakis(methanephosphonate) ligands, respectively. The apparent size and shape of Li+-free RBCs (red blood cells), studied by both scanning electron microscopy and Coulter counter methods, were unchanged by the presence of the above shift reagents at concentrations lower than 10 mM. However, Li+ incubation changed both the shape and size of RBCs. The rates of Na+-Li+ exchange in Li+-loaded RBCs measured by 7Li NMR spectroscopy in the presence of Dy(PPP);-, TI~(PPP),~o-r, D~(PcPcP),~w-e re significantly higher than the rates measured in the absence of shift reagents by atomic absorption or in the presence of DY(TTHA)~o-r DY(DOTP)~b-y 7Li NMR spectroscopy. 31P and I9F NMR measurements of the membrane potential of Li+-free RBCs revealed that the shift reagents studied (except for Dy(TTHA)”) do change the membrane potential, with the most negatively charged reagents having the largest effect. Thus, shift reagents must be used with caution in physiological NMR studies and in particular RBC applications. | URI: | https://hdl.handle.net/10316/10509 | ISSN: | 0020-1669 | DOI: | 10.1021/ic00345a014 | Rights: | openAccess |
Appears in Collections: | FCTUC Química - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Effects of Negatively Charged Shift Reagents on Red Blood Cell Morphology.pdf | 3.17 MB | Adobe PDF | View/Open |
SCOPUSTM
Citations
21
checked on May 1, 2023
WEB OF SCIENCETM
Citations
5
21
checked on May 2, 2023
Page view(s)
338
checked on Sep 25, 2024
Download(s)
435
checked on Sep 25, 2024
Google ScholarTM
Check
Altmetric
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.