Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/10472
Title: Surface Complexation of DNA with Insoluble Monolayers. Influence of Divalent Counterions
Authors: McLoughlin, D. 
Dias, R. 
Lindman, B. 
Cardenas, M. 
Nylander, T. 
Dawson, K. 
Miguel, M. 
Langevin, D. 
Issue Date: 1-Mar-2005
Publisher: American Chemical Society
Citation: Langmuir. 21:5 (2005) 1900-1907
Abstract: DNA interacts with insoluble monolayers made of cationic amphiphiles as well as with monolayers of zwitterionic lipids in the presence of divalent ions. Binding to dioctadecyldimethylammonium bromide (DODAB) or distearoyl-sn-glycero-3-phosphocholine (DSPC) monolayers in the presence of calcium is accompanied by monolayer expansion. For the positively charged DODAB monolayer, this causes a decrease of surface potential, while an increase is observed for the DSPC monolayers. Binding to dipalmitoyl-sn-glycero-3-phosphocholine preserves most of the liquid expanded−liquid condensed coexistence region. The liquid condensed domains adopt an elongated morphology in the presence of DNA, especially in the presence of calcium. The interaction of DNA with phospholipid monolayers is ion specific: the presence of calcium leads to a stronger interaction than magnesium and barium. These results were confirmed by bulk complexation studies.
URI: http://hdl.handle.net/10316/10472
ISSN: 0743-7463
DOI: 10.1021/la047700s
Rights: openAccess
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais

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