Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/103801
DC Field | Value | Language |
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dc.contributor.author | Lopes, Ines Rodrigues | - |
dc.contributor.author | Alcantara, Laura Maria | - |
dc.contributor.author | Silva, Ricardo Jorge | - |
dc.contributor.author | Josse, Jerome | - |
dc.contributor.author | Vega, Elena Pedrero | - |
dc.contributor.author | Cabrerizo, Ana Marina | - |
dc.contributor.author | Bonhomme, Melanie | - |
dc.contributor.author | Lopez, Daniel | - |
dc.contributor.author | Laurent, Frederic | - |
dc.contributor.author | Vandenesch, Francois | - |
dc.contributor.author | Mano, Miguel | - |
dc.contributor.author | Eulálio, Ana | - |
dc.date.accessioned | 2022-11-28T23:51:11Z | - |
dc.date.available | 2022-11-28T23:51:11Z | - |
dc.date.issued | 2022-11-22 | - |
dc.identifier.issn | 2041-1723 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/103801 | - |
dc.description.abstract | Staphylococcus aureus is increasingly recognized as a facultative intracellular pathogen, although the significance and pervasiveness of its intracellular lifestyle remain controversial. Here, we applied fluorescence microscopy-based infection assays and automated image analysis to profile the interaction of 191 S. aureus isolates from patients with bone/joint infections, bacteremia, and infective endocarditis, with four host cell types, at five times post-infection. This multiparametric analysis revealed that almost all isolates are internalized and that a large fraction replicate and persist within host cells, presenting distinct infection profiles in non-professional vs. professional phagocytes. Phenotypic clustering highlighted interesting sub-groups, including one comprising isolates exhibiting high intracellular replication and inducing delayed host death in vitro and in vivo. These isolates are deficient for the cysteine protease staphopain A. This study establishes S. aureus intracellular lifestyle as a prevalent feature of infection, with potential implications for the effective treatment of staphylococcal infections. | pt |
dc.description.sponsorship | We thank Martin J. Fraunholz (University of Würzburg) for providing the pLVTHM-H2B-BFP-IRES-mRFP-CWT plasmid, and Ian Monk (University of Melbourne, Australia) for the E. coli IM08B and IM01B strains. This work was supported by grants from the European Union’s Horizon 2020 research and innovation program (under the Marie Skłodowska-Curie grant agreement No 893942 to L.M.A), ERA-NET Infect-ERA StaphIN (031L0094, BMBF, Germany, to A.E.; Infect-ERA/0001/2015, FCT, Portugal, to M.M.; PCIN-2015-151, MINECO, Spain, to D.L.; and ANR 15-IFEC-0002-04, France, to F.L. and F.V), and the ERDF—European Regional Development Fund through COMPETE 2020 and Portuguese Foundation for Science and Technology (POCI-01-0145-FEDER-007440, UIDB/04539/2020, POCI-01-0145-FEDER-029999 to M.M. and A.E.) | pt |
dc.language.iso | eng | pt |
dc.publisher | Nature | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_CENTRO/PD/BD/146464/2019/PT/Deciphering host cell factors required for staphylococcus aureus infection | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_CENTRO/PD/BD/129294/2017/PT/Identification of novel regulators of cardiac ischemia-reperfussion injury using genome-wide CRISPR/Cas9 functional screening | pt |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/893942/EU/Functional high-throughput analysis of the role of microRNAs in regulating Staphylococcus aureus infection | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/3599-PPCDT/Infect-ERA/0001/2015/PT/Intracellular Staphylococcus aureus: deciphering bacterial and cellular factors involved in host cell invasion by clinically relevant strains to define new therapeutic approaches | pt |
dc.relation | info:eu-repo/grantAgreement/POCI-01-0145-FEDER-029999/PT | pt |
dc.relation | UIDB/04539/2020 | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID/NEU/04539/2013/PT | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject.mesh | Humans | pt |
dc.subject.mesh | Staphylococcus aureus | pt |
dc.subject.mesh | Microscopy | pt |
dc.subject.mesh | Life Style | pt |
dc.subject.mesh | Staphylococcal Infections | pt |
dc.subject.mesh | Bacteremia | pt |
dc.title | Microscopy-based phenotypic profiling of infection by Staphylococcus aureus clinical isolates reveals intracellular lifestyle as a prevalent feature | pt |
dc.type | article | - |
degois.publication.firstPage | 7174 | pt |
degois.publication.issue | 1 | pt |
degois.publication.title | Nature Communications | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1038/s41467-022-34790-9 | pt |
degois.publication.volume | 13 | pt |
dc.date.embargo | 2022-11-22 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology - CIBB | - |
crisitem.project.grantno | CNC. IBILI | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-6313-5119 | - |
crisitem.author.orcid | 0000-0003-1922-4824 | - |
crisitem.author.orcid | 0000-0002-7355-0674 | - |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
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s41467-022-34790-9.pdf | 7.58 MB | Adobe PDF | View/Open |
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