Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/103357
Title: Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta: Clues for Biotechnology
Authors: Rodrigo, Ana P.
Lopes, Ana
Pereira, Ricardo
Anjo, Sandra I. 
Manadas, Bruno 
Grosso, Ana R.
Baptista, Pedro V.
Fernandes, Alexandra R.
Costa, Pedro M.
Keywords: fluorescence; mucosecretions; UV light; ubiquitin conjugates; marine Annelida; redox status; proteome; mass spectrometry; poisonous secretions; transcriptomics
Issue Date: 25-Mar-2022
Project: FCT - PTDC/BTA-BTA/28650/2017 
FCT - grant SFRH/BD/109462/2015 
FCT - grant CEECIND/02699/2017 
UIDP/04378/2020 
UIDB/04378/2020 
UIDB/04539/2020 
UIDP/04539/2020 
POCI-01-0145-FEDER-402-022125 (ref.: ROTEIRO/0028/2013) 
Serial title, monograph or event: Marine Drugs
Volume: 20
Issue: 4
Abstract: The vast ocean holds many unexplored organisms with unique adaptive features that enable them to thrive in their environment. The secretion of fluorescent proteins is one of them, with reports on the presence of such compounds in marine annelids being scarce. The intertidal Eulalia sp. is an example. The worm secretes copious amounts of mucus, that when purified and concentrated extracts, yield strong fluorescence under UV light. Emission has two main maxima, at 400 nm and at 500 nm, with the latter responsible for the blue-greenish fluorescence. Combining proteomics and transcriptomics techniques, we identified ubiquitin, peroxiredoxin, and 14-3-3 protein as key elements in the mucus. Fluorescence was found to be mainly modulated by redox status and pH, being consistently upheld in extracts prepared in Tris-HCl buffer with reducing agent at pH 7 and excited at 330 nm. One of the proteins associated with the fluorescent signal was localized in secretory cells in the pharynx. The results indicate that the secretion of fluorescent proteinaceous complexes can be an important defense against UV for this dweller. Additionally, the internalization of fluorescent complexes by ovarian cancer cells and modulation of fluorescence of redox status bears important considerations for biotechnological application of mucus components as markers.
URI: https://hdl.handle.net/10316/103357
ISSN: 1660-3397
DOI: 10.3390/md20040224
Rights: openAccess
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais

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This item is licensed under a Creative Commons License Creative Commons