Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/10333
DC Field | Value | Language |
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dc.contributor.author | Guiomar, A. Jorge | - |
dc.contributor.author | Guthrie, James T. | - |
dc.contributor.author | Evans, Stephen D. | - |
dc.date.accessioned | 2009-06-25T08:13:24Z | - |
dc.date.available | 2009-06-25T08:13:24Z | - |
dc.date.issued | 1999-02-16 | - |
dc.identifier.citation | Langmuir. 15:4 (1999) 1198-1207 | en_US |
dc.identifier.issn | 0743-7463 | - |
dc.identifier.uri | https://hdl.handle.net/10316/10333 | - |
dc.description.abstract | Mixed self-assembled monolayers (SAMs) composed of a medium length, reactive, n-organothiol (11-mercaptoundecanoic acid) and a short length, unreactive, hydrophobic n-alkylthiol (7-heptanethiol) chemisorbed onto evaporated gold surfaces, were used to study the effect of the microenvironment on the structure and activity of immobilized glucose oxidase (GOX). The mixed SAMs were characterized by X-ray photoelectron spectroscopy, contact angle measurements, and cyclic voltammetry. The derivatization of the SAMs for the covalent attachment and the immobilized enzyme were studied by X-ray photoelectron spectroscopy. Quantitative analysis of the amide I band of the infrared spectra of GOX immobilized onto surfaces at the two attainable extremes of the hydrophilicity range available to us indicated that the percentage of β sheet increased with increasing hydrophilicity of the microenvironment. The specific activity of GOX was higher when the enzyme was immobilized onto the hydrophobic microenvironment. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | American Chemical Society | en_US |
dc.rights | openAccess | eng |
dc.title | Use of Mixed Self-Assembled Monolayers in a Study of the Effect of the Microenvironment on Immobilized Glucose Oxidase | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1021/la980974t | - |
item.openairetype | article | - |
item.fulltext | Com Texto completo | - |
item.languageiso639-1 | en | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
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Use of Mixed Self-Assembled Monolayers.pdf | 422.9 kB | Adobe PDF | View/Open |
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