Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/103317
DC Field | Value | Language |
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dc.contributor.author | Moniz, Inês | - |
dc.contributor.author | Ramalho-Santos, João | - |
dc.contributor.author | Branco, Ana F. | - |
dc.date.accessioned | 2022-11-04T11:50:50Z | - |
dc.date.available | 2022-11-04T11:50:50Z | - |
dc.date.issued | 2022-03-29 | - |
dc.identifier.issn | 1422-0067 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/103317 | - |
dc.description.abstract | Mesenchymal stem cells reside under precise hypoxic conditions that are paramount in determining cell fate and behavior (metabolism, proliferation, differentiation, etc.). In this work, we show that different oxygen tensions promote a distinct proliferative response and affect the biosynthetic demand and global metabolic profile of umbilical cord-mesenchymal stem cells (UC-MSCs). Using both gas-based strategies and CoCl2 as a substitute for the costly hypoxic chambers, we found that specific oxygen tensions influence the fate of UC-MSCs differently. While 5% O2 potentiates proliferation, stimulates biosynthetic pathways, and promotes a global hypermetabolic profile, exposure to <1% O2 contributes to a quiescent-like cell state that relies heavily on anaerobic glycolysis. We show that using CoCl2 as a hypoxia substitute of moderate hypoxia has distinct metabolic effects, when compared with gas-based strategies. The present study also highlights that, while severe hypoxia regulates global translation via mTORC1 modulation, its effects on survival-related mechanisms are mainly modulated through mTORC2. Therefore, the experimental conditions used in this study establish a robust and reliable hypoxia model for UC-MSCs, providing relevant insights into how stem cells are influenced by their physiological environment, and how different strategies of modulating hypoxia may influence experimental outcomes. | pt |
dc.language.iso | eng | pt |
dc.relation | CEECIND/00860/2018 | pt |
dc.relation | STEM@REST Project (CENTRO-01-0145-FEDER-028871) | pt |
dc.relation | CENTRO-01-0145-FEDER-000012- HealthyAging2020 | pt |
dc.relation | POCI-01-0145-FEDER-007440 | pt |
dc.relation | UID/NEU/04539/2020 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | mesenchymal stem cells | pt |
dc.subject | hypoxia | pt |
dc.subject | metabolism | pt |
dc.subject | mTOR | pt |
dc.subject | cobalt chloride | pt |
dc.subject.mesh | Cell Differentiation | pt |
dc.subject.mesh | Cell Hypoxia | pt |
dc.subject.mesh | Cell Proliferation | pt |
dc.subject.mesh | Cells, Cultured | pt |
dc.subject.mesh | Humans | pt |
dc.subject.mesh | Hypoxia | pt |
dc.subject.mesh | Oxygen | pt |
dc.subject.mesh | TOR Serine-Threonine Kinases | pt |
dc.subject.mesh | Mesenchymal Stem Cells | pt |
dc.title | Differential Oxygen Exposure Modulates Mesenchymal Stem Cell Metabolism and Proliferation through mTOR Signaling | pt |
dc.type | article | - |
degois.publication.firstPage | 3749 | pt |
degois.publication.issue | 7 | pt |
degois.publication.title | International Journal of Molecular Sciences | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3390/ijms23073749 | pt |
degois.publication.volume | 23 | pt |
dc.date.embargo | 2022-03-29 | * |
uc.date.periodoEmbargo | 0 | pt |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | Com Texto completo | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-0381-4101 | - |
crisitem.author.orcid | 0000-0002-1172-4018 | - |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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Differential-Oxygen-Exposure-Modulates-Mesenchymal-Stem-Cell-Metabolism-and-Proliferation-through-mTOR-SignalingInternational-Journal-of-Molecular-Sciences.pdf | 2.21 MB | Adobe PDF | View/Open |
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