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Título: | KPC-3-, GES-5-, and VIM-1-Producing Enterobacterales Isolated from Urban Ponds | Autor: | Teixeira, Pedro Pinto, Nuno Henriques, Isabel Tacão, Marta |
Palavras-chave: | antibiotic resistance; carbapenemases; Enterobacterales; urban aquatic environments | Data: | 2022 | Projeto: | UIDP/50017/2020 UIDB/50017/2020 LA/P/0094/2020 CFE-Centre for Functional Ecology-Science for People & the Planet’s Strategic Plan (UIDB/04004/2020) SFRH/BD/132046/2017 CEECIND/00977/2020 |
Título da revista, periódico, livro ou evento: | International Journal of Environmental Research and Public Health | Volume: | 19 | Número: | 10 | Resumo: | Carbapenems are antibiotics of pivotal importance in human medicine, the efficacy of which is threatened by the increasing prevalence of carbapenem-resistant Enterobacterales (CRE). Urban ponds may be reservoirs of CRE, although this hypothesis has been poorly explored. We assessed the proportion of CRE in urban ponds over a one-year period and retrieved 23 isolates. These were submitted to BOX-PCR, PFGE, 16S rDNA sequencing, antibiotic susceptibility tests, detection of carbapenemase-encoding genes, and conjugation assays. Isolates were affiliated with Klebsiella (n = 1), Raoultella (n = 11), Citrobacter (n = 8), and Enterobacter (n = 3). Carbapenemase-encoding genes were detected in 21 isolates: blaKPC (n = 20), blaGES-5 (n = 6), and blaVIM (n = 1), with 7 isolates carrying two carbapenemase genes. Clonal isolates were collected from different ponds and in different campaigns. Citrobacter F6, Raoultella N9, and Enterobacter N10 were predicted as pathogens from whole-genome sequence analysis, which also revealed the presence of several resistance genes and mobile genetic elements. We found that blaKPC-3 was located on Tn4401b (Citrobacter F6 and Enterobacter N10) or Tn4401d (Raoultella N9). The former was part of an IncFIA-FII pBK30683-like plasmid. In addition, blaGES-5 was in a class 3 integron, either chromosomal (Raoultella N9) or plasmidic (Enterobacter N10). Our findings confirmed the role of urban ponds as reservoirs and dispersal sites for CRE. | URI: | https://hdl.handle.net/10316/103302 | ISSN: | 1660-4601 | DOI: | 10.3390/ijerph19105848 | Direitos: | openAccess |
Aparece nas coleções: | I&D CFE - Artigos em Revistas Internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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KPC3-GES5-and-VIM1Producing-Enterobacterales-Isolated-from-Urban-PondsInternational-Journal-of-Environmental-Research-and-Public-Health.pdf | 1.66 MB | Adobe PDF | Ver/Abrir |
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