Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/100110
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dc.contributor.authorCaldeira, G. L.-
dc.contributor.authorInácio, A.-
dc.contributor.authorBeltrão, N.-
dc.contributor.authorBarreto, C. A. V.-
dc.contributor.authorRodrigues, M. V.-
dc.contributor.authorRondão, T.-
dc.contributor.authorMacedo, R.-
dc.contributor.authorGouveia, R. P.-
dc.contributor.authorEdfawy, M.-
dc.contributor.authorGuedes, J.-
dc.contributor.authorCruz, B.-
dc.contributor.authorLouros, S. R.-
dc.contributor.authorMoreira, I. S.-
dc.contributor.authorPeça, J.-
dc.contributor.authorCarvalho, A. L.-
dc.date.accessioned2022-05-06T14:49:59Z-
dc.date.available2022-05-06T14:49:59Z-
dc.date.issued2022-03-07-
dc.identifier.issn1359-4184pt
dc.identifier.issn1476-5578pt
dc.identifier.urihttps://hdl.handle.net/10316/100110-
dc.description.abstractMutations linked to neurodevelopmental disorders, such as intellectual disability (ID), are frequently found in genes that encode for proteins of the excitatory synapse. Transmembrane AMPA receptor regulatory proteins (TARPs) are AMPA receptor auxiliary proteins that regulate crucial aspects of receptor function. Here, we investigate a mutant form of the TARP family member stargazin, described in an ID patient. Molecular dynamics analyses predicted that the ID-associated stargazin variant, V143L, weakens the overall interface of the AMPAR:stargazin complex and impairs the stability of the complex. Knock-in mice harboring the V143L stargazin mutation manifest cognitive and social deficits and hippocampal synaptic transmission defects, resembling phenotypes displayed by ID patients. In the hippocampus of stargazin V143L mice, CA1 neurons show impaired spine maturation, abnormal synaptic transmission and long-term potentiation specifically in basal dendrites, and synaptic ultrastructural alterations. These data suggest a causal role for mutated stargazin in the pathogenesis of ID and unveil a new role for stargazin in the development and function of hippocampal synapses.pt
dc.description.sponsorshipThis work was supported by a NARSAD Independent Investigator Grant (#23151) and a NARSAD Young Investigator Grant (#20733) from the Brain and Behavior Research Foundation, by a research grant from the Jérôme Lejeune Foundation (#1530), by “la Caixa” Foundation (ID 100010434), and FCT, I.P under the project code LCF/PR/HP20/52300003, by a Marie Curie Integration Grant (618525), by a Bial Foundation Grant (266/2016), by national funds through the Portuguese Science and Technology Foundation (FCT: UID/NEU/04539/2013, UIDB/04539/2020, POCI-01-0145-FEDER-28541, POCI-01-0145-FEDER-016682, PTDC/QUI-OUT/32243/2017 and CPCA/A0/7302/2020), and by the European Regional Development Fund (ERDF), through the Centro 2020 Regional Operational Programme, under project CENTRO-01-0145-FEDER-000008:BrainHealth 2020. GLC, NB, MVR, ME and CAVB were supported by FCT through Ph.D. scholarships SFRH/BD/51962/2012, SFRH/BD/144881/2019, SFRH/BD/129236/2017, SFRH/BD/51958/2012 and SFRH/BD/145457/2019, respectively. ASI and JG were supported by FCT through Postdoctoral fellowship SFRH/BPD122299/2016 and SFRH/BPD/120611/2016, respectively. RPG and RM received support from FCT/DGES, under the program “Verão com Ciência”.pt
dc.language.isoengpt
dc.publisherSpringer Naturept
dc.relationUID/NEU/04539/2013pt
dc.relationUIDB/04539/2020pt
dc.relationPOCI-01-0145-FEDER-28541pt
dc.relationPOCI-01-0145- FEDER-016682pt
dc.relationPTDC/QUI-OUT/32243/2017pt
dc.relationCPCA/A0/7302/2020pt
dc.relationCENTRO-01-0145-FEDER-000008:BrainHealth 2020pt
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH/BD/51962/2012/PTpt
dc.relationinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH/BD/144881/2019/PT/Cerebellar dysfunction in neuropsychiatric disorders: insights from human mutations in Stargazinpt
dc.relationinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH/BD/129236/2017/PT/STARGAZIN AS A REGULATOR OF M-CURRENTS IN THE CENTRAL NERVOUS SYSTEMpt
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH/2FBD/2F51958/2F2012/PTpt
dc.relationinfo:eu-repo/grantAgreement/FCT/OE/SFRH/BD/145457/2019/PT/A computational approach to the structural and dynamical characterization of the ghrelin receptor function and mechanismpt
dc.relationinfo:eu-repo/grantAgreement/FCT/FARH/SFRH/BPD/122299/2016/PT/COGNITIVE DISORDERS AS DEFECTS IN SYNAPTIC FUNCTION: INSIGHT FROM HUMAN MUTATIONS IN THE CACNG2 GENEpt
dc.relationinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH/BPD/120611/2016/PT/Dissecting the role of metabotropic glutamate receptor sorting proteins in autism spectrum disorderspt
dc.rightsopenAccesspt
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt
dc.titleAberrant hippocampal transmission and behavior in mice with a stargazin mutation linked to intellectual disabilitypt
dc.typearticle-
degois.publication.titleMolecular Psychiatrypt
dc.peerreviewedyespt
dc.identifier.doi10.1038/s41380-022-01487-wpt
dc.date.embargo2022-03-07*
uc.date.periodoEmbargo0pt
item.openairetypearticle-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.fulltextCom Texto completo-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0003-1454-8299-
crisitem.author.orcid0000-0001-8368-6666-
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais
IIIUC - Artigos em Revistas Internacionais
FCTUC Ciências da Vida - Artigos em Revistas Internacionais
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