Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/95119
Title: Rational selection of hidden epitopes for a molecularly imprinted electrochemical sensor in the recognition of heat-denatured dengue NS1 protein
Authors: Silva, Matheus Siqueira
Tavares, Ana Patricia Moreira
Coelho, Luiz Felipe Leomil
Dias, Lígia Ely Morganti Ferreira
Chura-Chambi, Rosa Maria
Guimarães da Fonseca, Flávio
Sales, Maria Goreti Ferreira 
Figueiredo, Eduardo Costa
Keywords: DENV; Epitope; Label-free; Molecularly imprinted polymers; NS1; Sensor
Issue Date: 8-Jun-2021
Publisher: Elsevier
Serial title, monograph or event: Biosensors and Biolectronics
Volume: 191
Abstract: Rational selection of predicted peptides to be employed as templates in molecular imprinting was carried out for the heat-denatured non-structural protein 1 (NS1) of dengue virus (DENV). Conservation analysis among 301 sequences of Brazilian isolates of DENV and zika virus (ZIKV) NS1 was carried out by UniProtKB, and peptide selection was based on in silico data of the conservational, structural and immunogenic properties of the sequences. The selected peptide (from dengue 1 NS1) was synthesized and employed as a template in the electropolymerization of polyaminophenol-imprinted films on the surface of carbon screen-printed electrodes. Heat denaturation of the protein was carried out prior to analysis, in order to expose its internal hidden epitopes. After removal of the template, the molecularly imprinted cavities were able to rebind to the whole denatured protein as determined by electrochemical impedance spectroscopy. This label-free sensor was efficient to distinguish the NS1 of DENV from the NS1 of ZIKV. Additionally, the sensor was also selective for dengue NS1, in comparison with human serum immunoglobulin G and human serum albumin. Additionally, the device was able to detect the DENV NS1 at concentrations from 50 to 200 μg L-1 (RSD below 5.04%, r = 0.9678) in diluted human serum samples. The calculated LOD and LOQ were, respectively, 29.3 and 88.7 μg L-1 and each sensor could be used for six sequential cycles with the same performance.
URI: https://hdl.handle.net/10316/95119
ISSN: 09565663
DOI: 10.1016/j.bios.2021.113419
Rights: openAccess
Appears in Collections:FCTUC Eng.Química - Artigos em Revistas Internacionais

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