Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/92394
Title: Vulnerability of progeroid smooth muscle cells to biomechanical forces is mediated by MMP13
Authors: Pitrez, Patrícia R. 
Estronca, Luís Miguel Beicinha Branco 
Monteiro, Luís Miguel Marques 
Colell, Guillem
Vazão, Helena 
Santinha, Deolinda da Conceição Ribafeita 
Harhouri, Karim
Thornton, Daniel
Navarro, Claire
Egesipe, Anne-Laure
Carvalho, Tania Rafaela Vale 
Santos, Rodrigo L. dos
Lévy, Nicolas 
Smith, James C.
Magalhães, João Pedro de
Ori, Alessandro
Bernardo, Andreia
De Sandre-Giovannoli, Annachiara
Nissan, Xavier
Rosell, Anna
Ferreira, Lino 
Issue Date: 2020
Publisher: Nature
Serial title, monograph or event: Nature Communications
Volume: 11
Issue: 1
Abstract: Hutchinson-Gilford Progeria Syndrome (HGPS) is a premature aging disease in children that leads to early death. Smooth muscle cells (SMCs) are the most affected cells in HGPS individuals, although the reason for such vulnerability remains poorly understood. In this work, we develop a microfluidic chip formed by HGPS-SMCs generated from induced pluripotent stem cells (iPSCs), to study their vulnerability to flow shear stress. HGPS-iPSC SMCs cultured under arterial flow conditions detach from the chip after a few days of culture; this process is mediated by the upregulation of metalloprotease 13 (MMP13). Importantly, double-mutant LmnaG609G/G609GMmp13-/- mice or LmnaG609G/G609GMmp13+/+ mice treated with a MMP inhibitor show lower SMC loss in the aortic arch than controls. MMP13 upregulation appears to be mediated, at least in part, by the upregulation of glycocalyx. Our HGPS-SMCs chip represents a platform for developing treatments for HGPS individuals that may complement previous pre-clinical and clinical treatments.
URI: https://hdl.handle.net/10316/92394
ISSN: 2041-1723
DOI: 10.1038/s41467-020-17901-2
Rights: openAccess
Appears in Collections:UC Bibliotecas - Artigos em Revistas Internacionais

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