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Title: Flexible nanofilms coated with aligned piezoelectric microfibers preserve the contractility of cardiomyocytes
Authors: Gouveia, P José
Rosa, S
Ricotti, L
Abecasis, B
Almeida, H V
Monteiro, L. R. 
Nunes, J
Carvalho, F Sofia
Serra, M
Luchkin, S
Kholkin, A Leonidovitch
Alves, P Marques
Oliveira, P Jorge
Carvalho, R
Menciassi, A
das Neves, R Pires
Ferreira, L Silva
Keywords: Cardiac tissue engineering; Cardiotoxicity; Electrospun fibers; Nanofilms; Piezoelectric materials
Issue Date: Sep-2017
Publisher: Elsevier
Project: This work was supported by funds from FEDER through COMPETE program and Fundação para a Ciência e a Tecnologia (FCT) (EXPL/DTP-FTO/0570/2012, MITPTB/ ECE/0013/2013 to S. R. and L.F., and PTDC/SAU-ENB/113696/2009 to R.P.N.; SFRH/BD/51197/2010 and SFRH/BPD/79323/2011 to P.G. and S.R., respectively) as well as COMPETE program for the project “Stem cell based platforms for Regenerative and Therapeutic Medicine” (Centro-07-ST24-FEDER-002008). The authors also would like to acknowledge the help of Tommaso Mazzochi for the finite model simulations. 
Serial title, monograph or event: Biomaterials
Volume: 139
Abstract: The use of engineered cardiac tissue for high-throughput drug screening/toxicology assessment remains largely unexplored. Here we propose a scaffold that mimics aspects of cardiac extracellular matrix while preserving the contractility of cardiomyocytes. The scaffold is based on a poly(caprolactone) (PCL) nanofilm with magnetic properties (MNF, standing for magnetic nanofilm) coated with a layer of piezoelectric (PIEZO) microfibers of poly(vinylidene fluoride-trifluoroethylene) (MNF+PIEZO). The nanofilm creates a flexible support for cell contraction and the aligned PIEZO microfibers deposited on top of the nanofilm creates conditions for cell alignment and electrical stimulation of the seeded cells. Our results indicate that MNF+PIEZO scaffold promotes rat and human cardiac cell attachment and alignment, maintains the ratio of cell populations overtime, promotes cell-cell communication and metabolic maturation, and preserves cardiomyocyte (CM) contractility for at least 12 days. The engineered cardiac construct showed high toxicity against doxorubicin, a cardiotoxic molecule, and responded to compounds that modulate CM contraction such as epinephrine, propranolol and heptanol.
ISSN: 01429612
DOI: 10.1016/j.biomaterials.2017.05.048
Rights: openAccess
Appears in Collections:UC Bibliotecas - Artigos em Revistas Internacionais

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