Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/88171
Title: | A light-triggerable nanoparticle library for the controlled release of non-coding RNAs | Authors: | Josephine, Blersch Vitor, Francisco Rebelo, Catarina Adrian, Jimenez-Balsa Antunes, Helena Carlo, Gonzato Pinto, Sandra Simões, Susana Klaus, Liedl Karsten, Haupt Ferreira, Lino |
Issue Date: | 14-Nov-2019 | Publisher: | Wiley | Serial title, monograph or event: | Angewandte Chemie International Edition | Abstract: | RNA-based therapies offer a wide range of therapeutic interventions including for the treatment of skin diseases; however, the strategies to deliver efficiently these biomolecules are still limited due to obstacles related to the cellular uptake and cytoplasmic delivery. Herein, we synthesized a triggerable polymeric nanoparticle (NP) library composed by 160 formulations, presenting physico-chemical diversity and differential responsiveness to light. Six formulations were more efficient (up to 500%) than commercial Lipofectamine in gene knockdown activity. These formulations had differential internalization by skin cells and the endosomal escape was rapid (minutes range) as shown by the recruitment of galectin-8. The NPs were effective in the release of siRNA and miRNA. Acute skin wounds treated with the top hit NP complexed with miRNA-150-5p healed faster than wounds treated with scramble miRNA. Light-activatable NPs offer a new strategy to deliver topically non-coding RNAs. | URI: | https://hdl.handle.net/10316/88171 | DOI: | 10.1002/anie.201911398 | Rights: | openAccess |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
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Manuscript.pdf | 1.68 MB | Adobe PDF | View/Open | |
Supporting Information.pdf | 4.72 MB | Adobe PDF | View/Open |
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