Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/8491
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Santos, T. Almeida | - |
dc.contributor.author | Dias, C. | - |
dc.contributor.author | Henriques, P. | - |
dc.contributor.author | Brito, R. | - |
dc.contributor.author | Barbosa, A. | - |
dc.contributor.author | Regateiro, F. | - |
dc.contributor.author | Santos, A. Almeida | - |
dc.date.accessioned | 2009-02-17T10:51:32Z | - |
dc.date.available | 2009-02-17T10:51:32Z | - |
dc.date.issued | 2003 | - |
dc.identifier.citation | Journal of Assisted Reproduction and Genetics. 20:3 (2003) 122-130 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/8491 | - |
dc.description.abstract | Purpose: Spontaneous parthenogenetically activated noninseminated oocytes and failed fertilized oocytes after ART activated by puromycin were studied to assess cleavage ability and the cytogenetic constitution of the resulting embryos. Methods: Failed fertilized oocytes were exposed to puromycin, and whenever activation occurred, they were further cultured until arrest of development. FISH was used to assess the ploidy of spontaneous (group A) and induced parthenotes (group B). Results: The mean number of oocytes exposed to puromycin and the percentage and type of activation were identical in IVF and ICSI patients. The more frequent types of activation were one or two pronuclei and one polar body suggesting that retention of the second polar body is a common event after parthenogenetic activation. Conclusions: Retention of the second polar body and chromosome malsegregation were observed after parthenogenetic activation, either spontaneous or induced by puromycin. This means that using parthenogenetic embryos for stem cell research will require great care and attention. | pt |
dc.description.sponsorship | This work was supported by a grant from the Reitoria da Universidade de Coimbra, Portugal. | pt |
dc.language.iso | eng | pt |
dc.publisher | Plenum Publishing Corporation | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | FISH | pt |
dc.subject | Human oocytes | pt |
dc.subject | Parthenogenetic activation | pt |
dc.subject | Puromycin | pt |
dc.subject | Stem cell production | pt |
dc.title | Cytogenetic Analysis of Spontaneously Activated Noninseminated Oocytes and Parthenogenetically Activated Failed Fertilized Human Oocytes—Implications for the Use of Primate Parthenotes for Stem Cell Production | pt |
dc.type | article | en_US |
degois.publication.firstPage | 122 | pt |
degois.publication.lastPage | 130 | pt |
degois.publication.issue | 3 | pt |
degois.publication.title | Journal of Assisted Reproduction and Genetic | pt |
dc.relation.publisherversion | https://link.springer.com/article/10.1023%2FA%3A1022630924236 | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1023/A:1022630924236 | pt |
degois.publication.volume | 20 | pt |
dc.date.embargo | 2003-01-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.grantfulltext | open | - |
item.fulltext | Com Texto completo | - |
item.openairetype | article | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Faculty of Medicine | - |
crisitem.author.parentdept | University of Coimbra | - |
crisitem.author.orcid | 0000-0001-7423-2996 | - |
Appears in Collections: | FMUC Medicina - Artigos em Revistas Internacionais |
SCOPUSTM
Citations
21
checked on Apr 15, 2024
WEB OF SCIENCETM
Citations
5
21
checked on Apr 2, 2024
Page view(s) 50
574
checked on Apr 23, 2024
Download(s)
253
checked on Apr 23, 2024
Google ScholarTM
Check
Altmetric
Altmetric
This item is licensed under a Creative Commons License