Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/8430
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Perdigoto, Rui | - |
dc.contributor.author | Rodrigues, Tiago B. | - |
dc.contributor.author | Furtado, Alexandre L. | - |
dc.contributor.author | Porto, Armando | - |
dc.contributor.author | Geraldes, Carlos F. G. C. | - |
dc.contributor.author | Jones, John G. | - |
dc.date.accessioned | 2009-02-09T14:54:42Z | - |
dc.date.available | 2009-02-09T14:54:42Z | - |
dc.date.issued | 2003 | en_US |
dc.identifier.citation | NMR in Biomedicine. 16:4 (2003) 189-198 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/8430 | - |
dc.description.abstract | Glucose metabolism in five healthy subjects fasted for 16 h was measured with a combination of [U-13C]glucose and 2H2O tracers. Phenylbutyric acid was also provided to sample hepatic glutamine for the presence of 13C-isotopomers derived from the incorporation of [U-13C]glucose products into the hepatic Krebs cycle. Glucose production (GP) was quantified by 13C NMR analysis of the monoacetone derivative of plasma glucose following a primed infusion of [U-13C]glucose and provided reasonable estimates (1.90 ± 0.19 mg/kg/min with a range of 1.60-2.15 mg/kg/min). The same derivative yielded measurements of plasma glucose 2H-enrichment from 2H2O by 2H NMR from which the contribution of glycogenolytic and gluconeogenic fluxes to GP was obtained (0.87 ± 0.14 and 1.03 ± 0.10 mg/kg/min, respectively). Hepatic glutamine 13C-isotopomers representing multiply-enriched oxaloacetate and [U-13C]acetyl-CoA were identified as multiplets in the 13C NMR signals of the glutamine moiety of urinary phenylacetylglutamine, demonstrating entry of the [U-13C]glucose tracer into both oxidative and anaplerotic pathways of the hepatic Krebs cycle. These isotopomers contributed 0.1-0.2% excess enrichment to carbons 2 and 3 and sim0.05% to carbon 4 of glutamine. Copyright © 2003 John Wiley & Sons, Ltd. | en_US |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.title | Integration of [U-13C]glucose and 2H2O for quantification of hepatic glucose production and gluconeogenesis | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1002/nbm.826 | en_US |
uc.controloAutoridade | Sim | - |
item.grantfulltext | open | - |
item.fulltext | Com Texto completo | - |
item.openairetype | article | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
crisitem.author.researchunit | CQC - Coimbra Chemistry Centre | - |
crisitem.author.parentresearchunit | Faculty of Sciences and Technology | - |
crisitem.author.orcid | 0000-0002-0837-8329 | - |
crisitem.author.orcid | 0000-0002-3745-3885 | - |
Appears in Collections: | FMUC Medicina - Artigos em Revistas Internacionais |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.